Whole transcriptome analysis of the fasting and fed Burmese python heart: insights into extreme physiological cardiac adaptation

Wall, C. E., Cozza, S., Riquelme, C. A., McCombie, W. R., Heimiller, J. K., Marr, T. G., Leinwand, L. A. (2011) Whole transcriptome analysis of the fasting and fed Burmese python heart: insights into extreme physiological cardiac adaptation. Physiological Genomics, 43 (2). pp. 69-76. ISSN 1094-8341

URL: http://www.ncbi.nlm.nih.gov/pubmed/21045117
DOI: 10.1152/physiolgenomics.00162.2010

Abstract

Wall CE, Cozza S, Riquelme CA, McCombie WR, Heimiller JK, Marr TG, Leinwand LA. Whole transcriptome analysis of the fasting and fed Burmese python heart: insights into extreme physiological cardiac adaptation. Physiol Genomics 43: 69-76, 2011. First published November 2, 2010; doi:10.1152/physiolgenomics.00162.2010.-The infrequently feeding Burmese python (Python molurus) experiences significant and rapid postprandial cardiac hypertrophy followed by regression as digestion is completed. To begin to explore the molecular mechanisms of this response, we have sequenced and assembled the fasted and postfed Burmese python heart transcriptomes with Illumina technology using the chicken (Gallus gallus) genome as a reference. In addition, we have used RNA-seq analysis to identify differences in the expression of biological processes and signaling pathways between fasted, 1 day postfed (DPF), and 3 DPF hearts. Out of a combined transcriptome of similar to 2,800 mRNAs, 464 genes were differentially expressed. Genes showing differential expression at 1 DPF compared with fasted were enriched for biological processes involved in metabolism and energetics, while genes showing differential expression at 3 DPF compared with fasted were enriched for processes involved in biogenesis, structural remodeling, and organization. Moreover, we present evidence for the activation of physiological and not pathological signaling pathways in this rapid, novel model of cardiac growth in pythons. Together, our data provide the first comprehensive gene expression profile for a reptile heart.

Item Type: Paper
Uncontrolled Keywords: Illumina RNA-Seq RNA-SEQ HYPERTROPHY QUANTIFICATION MUSCLE MODEL
Subjects: bioinformatics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > transcriptomes
CSHL Authors:
Communities: CSHL labs > McCombie lab
Depositing User: Matt Covey
Date Deposited: 06 Feb 2013 16:28
Last Modified: 06 Feb 2013 16:28
PMCID: PMC3026562
Related URLs:
URI: http://repository.cshl.edu/id/eprint/27170

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