Comparative functional genomics of the fission yeasts

Rhind, N., Chen, Z., Yassour, M., Thompson, D. A., Haas, B. J., Habib, N., Wapinski, I., Roy, S., Lin, M. F., Heiman, D. I., Young, S. K., Furuya, K., Guo, Y., Pidoux, A., Chen, H. M., Robbertse, B., Goldberg, J. M., Aoki, K., Bayne, E. H., Berlin, A. M., Desjardins, C. A., Dobbs, E., Dukaj, L., Fan, L., FitzGerald, M. G., French, C., Gujja, S., Hansen, K., Keifenheim, D., Levin, J. Z., Mosher, R. A., Muller, C. A., Pfiffner, J., Priest, M., Russ, C., Smialowska, A., Swoboda, P., Sykes, S. M., Vaughn, M., Vengrova, S., Yoder, R., Zeng, Q., Allshire, R., Baulcombe, D., Birren, B. W., Brown, W., Ekwall, K., Kellis, M., Leatherwood, J., Levin, H., Margalit, H., Martienssen, R., Nieduszynski, C. A., Spatafora, J. W., Friedman, N., Dalgaard, J. Z., Baumann, P., Niki, H., Regev, A., Nusbaum, C. (May 2011) Comparative functional genomics of the fission yeasts. Science, 332 (6032). pp. 930-6. ISSN 1095-9203 (Electronic)0036-8075 (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/21511999
DOI: 10.1126/science.1203357

Abstract

The fission yeast clade--comprising Schizosaccharomyces pombe, S. octosporus, S. cryophilus, and S. japonicus--occupies the basal branch of Ascomycete fungi and is an important model of eukaryote biology. A comparative annotation of these genomes identified a near extinction of transposons and the associated innovation of transposon-free centromeres. Expression analysis established that meiotic genes are subject to antisense transcription during vegetative growth, which suggests a mechanism for their tight regulation. In addition, trans-acting regulators control new genes within the context of expanded functional modules for meiosis and stress response. Differences in gene content and regulation also explain why, unlike the budding yeast of Saccharomycotina, fission yeasts cannot use ethanol as a primary carbon source. These analyses elucidate the genome structure and gene regulation of fission yeast and provide tools for investigation across the Schizosaccharomyces clade.

Item Type: Paper
Uncontrolled Keywords: Centromere genetics physiology ultrastructure DNA Transposable Elements Evolution Molecular Gene Expression Profiling Gene Expression Regulation Fungal Genes Mating Type Fungal Genome Fungal Genomics Glucose metabolism Meiosis Molecular Sequence Annotation Molecular Sequence Data Phylogeny RNA Antisense genetics RNA Fungal genetics RNA Small Interfering genetics RNA Untranslated genetics Regulatory Elements Transcriptional Schizosaccharomyces genetics growth & development metabolism Schizosaccharomyces pombe Proteins genetics metabolism Sequence Analysis DNA Species Specificity Transcription Factors genetics metabolism Transcription Genetic
Subjects: bioinformatics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > genomes
organism description > yeast
CSHL Authors:
Communities: CSHL labs > Martienssen lab
CSHL Cancer Center Shared Resources > DNA Sequencing Service
Depositing User: Matt Covey
Date: 20 May 2011
Date Deposited: 06 Feb 2013 21:34
Last Modified: 26 Dec 2014 19:46
PMCID: PMC3131103
Related URLs:
URI: http://repository.cshl.edu/id/eprint/27162

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