Simultaneous assessment of rodent behavior and neurochemistry using a miniature positron emission tomograph

Schulz, D., Southekal, S. S., Junnarkar, S. S., Pratte, J. F., Purschke, M. L., Stoll, S. P., Ravindranath, B., Maramraju, S. H., Krishnamoorthy, S., Henn, F. A., O'Connor, P., Woody, C. L., Schlyer, D. J., Vaska, P. (April 2011) Simultaneous assessment of rodent behavior and neurochemistry using a miniature positron emission tomograph. Nature Methods, 8 (4). pp. 347-352. ISSN 15487091 (ISSN)

URL: http://www.ncbi.nlm.nih.gov/pubmed/21399637
DOI: 10.1038/nmeth.1582

Abstract

Positron emission tomography (PET) neuroimaging and behavioral assays in rodents are widely used in neuroscience. PET gives insights into the molecular processes of neuronal communication, and behavioral methods analyze the actions that are associated with such processes. These methods have not been directly integrated, because PET studies in animals have until now required general anesthesia to immobilize the subject, which precludes behavioral studies. We present a method for imaging awake, behaving rats with PET that allows the simultaneous study of behavior. Key components include the 'rat conscious animal PET' or RatCAP, a miniature portable PET scanner that is mounted on the rat's head, a mobility system that allows considerable freedom of movement, radiotracer administration techniques and methods for quantifying behavior and correlating the two data sets. The simultaneity of the PET and behavioral data provides a multidimensional tool for studying the functions of different brain regions and their molecular constituents. © 2011 Nature America, Inc. All rights reserved.

Item Type: Paper
Uncontrolled Keywords: tracer animal behavior animal experiment article brain region computed tomography scanner correlation analysis female molecular imaging neurochemistry nonhuman positron emission tomography priority journal rat rodent Animalia Rattus Rodentia
Subjects: organism description > animal
organism description > animal behavior
organism description > animal > mammal > rodent > rat
organism description > animal > mammal > rodent > rat

organism description > animal > mammal > rodent
CSHL Authors:
Communities: CSHL labs > Henn lab
Depositing User: Matt Covey
Date: April 2011
Date Deposited: 07 Feb 2013 16:14
Last Modified: 07 Feb 2013 16:14
Related URLs:
URI: http://repository.cshl.edu/id/eprint/27132

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