Sequencing the Connectome

Zador, A. M., Dubnau, J., Oyibo, H. K., Zhan, H., Cao, G., Peikon, I. D. (2012) Sequencing the Connectome. PLoS Biology, 10 (10). ISSN 1544-9173

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URL: http://www.ncbi.nlm.nih.gov/pubmed/23109909
DOI: 10.1371/journal.pbio.1001411

Abstract

Connectivity determines the function of neural circuits. Historically, circuit mapping has usually been viewed as a problem of microscopy, but no current method can achieve high-throughput mapping of entire circuits with single neuron precision. Here we describe a novel approach to determining connectivity. We propose BOINC ("barcoding of individual neuronal connections"), a method for converting the problem of connectivity into a form that can be read out by high-throughput DNA sequencing. The appeal of using sequencing is that its scale--sequencing billions of nucleotides per day is now routine--is a natural match to the complexity of neural circuits. An inexpensive high-throughput technique for establishing circuit connectivity at single neuron resolution could transform neuroscience research.

Item Type: Paper
Subjects: Investigative techniques and equipment
Investigative techniques and equipment > assays
organs, tissues, organelles, cell types and functions > organs types and functions > brain
organs, tissues, organelles, cell types and functions > cell types and functions > cell types
organs, tissues, organelles, cell types and functions > cell types and functions > cell types
organs, tissues, organelles, cell types and functions > cell types and functions > cell types

organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons

organs, tissues, organelles, cell types and functions
Investigative techniques and equipment > whole exome sequencing
Investigative techniques and equipment > assays > whole exome sequencing
CSHL Authors:
Communities: CSHL Post Doctoral Fellows
CSHL labs > Dubnau lab
CSHL labs > Zador lab
Watson School > Publications
Highlight: Dubnau, Josh T.
Depositing User: Matt Covey
Date Deposited: 17 Jan 2013 17:21
Last Modified: 22 Sep 2014 20:49
PMCID: PMC3479097
Related URLs:
URI: http://repository.cshl.edu/id/eprint/27091

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