Ectopic expression of a maize calreticulin mitigates calcium deficiency-like disorders in sCAX1-expressing tobacco and tomato

Wu, Q., Shigaki, T., Han, J. S., Kim, C. K., Hirschi, K. D., Park, S. (December 2012) Ectopic expression of a maize calreticulin mitigates calcium deficiency-like disorders in sCAX1-expressing tobacco and tomato. Plant Molecular Biology, 80 (6). pp. 609-619.

URL: http://www.ncbi.nlm.nih.gov/pubmed/23007728
DOI: 10.1007/s11103-012-9970-6

Abstract

Deregulated expression of an Arabidopsis H⁺/Ca²⁺ antiporter (sCAX1) in agricultural crops increases total calcium (Ca²⁺) but may result in yield losses due to Ca²⁺ deficiency-like symptoms. Here we demonstrate that co-expression of a maize calreticulin (CRT, a Ca²⁺ binding protein located at endoplasmic reticulum) in sCAX1-expressing tobacco and tomato plants mitigated these adverse effects while maintaining enhanced Ca²⁺ content. Co-expression of CRT and sCAX1 could alleviate the hypersensitivity to ion imbalance in tobacco plants. Furthermore, blossom-end rot (BER) in tomato may be linked to changes in CAX activity and enhanced CRT expression mitigated BER in sCAX1 expressing lines. These findings suggest that co-expressing Ca²⁺ transporters and binding proteins at different intracellular compartments can alter the content and distribution of Ca²⁺ within the plant matrix.

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
organism description > plant > maize
organism description > plant
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > transgenic plants
CSHL Authors:
Communities: CSHL Post Doctoral Fellows
CSHL labs > Jackson lab
Depositing User: Matt Covey
Date: December 2012
Date Deposited: 17 Jan 2013 19:52
Last Modified: 03 May 2013 13:26
Related URLs:
URI: http://repository.cshl.edu/id/eprint/27081

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