Serial two-photon tomography for automated ex vivo mouse brain imaging

Ragan, T., Kadiri, L. R., Venkataraju, K. U., Bahlmann, K., Sutin, J., Taranda, J., Arganda-Carreras, I., Kim, Y., Seung, H. S., Osten, P. (2012) Serial two-photon tomography for automated ex vivo mouse brain imaging. Nature Methods, 9 (3). pp. 255-258. ISSN 15487091 (ISSN)

URL: http://www.ncbi.nlm.nih.gov/pubmed/22245809
DOI: 10.1038/nmeth.1854

Abstract

Here we describe an automated method, named serial two-photon (STP) tomography, that achieves high-throughput fluorescence imaging of mouse brains by integrating two-photon microscopy and tissue sectioning. STP tomography generates high-resolution datasets that are free of distortions and can be readily warped in three dimensions, for example, for comparing multiple anatomical tracings. This method opens the door to routine systematic studies of neuroanatomy in mouse models of human brain disorders.

Item Type: Paper
Subjects: Investigative techniques and equipment
organism description > animal
organs, tissues, organelles, cell types and functions > organs types and functions > brain
Investigative techniques and equipment > microscopy > flourescence microscopy
Investigative techniques and equipment > imaging
organism description > animal > mammal
Investigative techniques and equipment > microscopy
organism description > animal > mammal > rodent > mouse
organs, tissues, organelles, cell types and functions > organs types and functions
organs, tissues, organelles, cell types and functions
organism description > animal > mammal > rodent
Investigative techniques and equipment > microscopy > flourescence microscopy > two-photon excitation microscopy
CSHL Authors:
Communities: CSHL Post Doctoral Fellows
CSHL labs > Osten lab
Stanley Institute for Cognitive Genomics
Highlight: Osten, Pavel
Depositing User: Matt Covey
Date Deposited: 29 Jan 2013 17:29
Last Modified: 06 Nov 2015 20:58
PMCID: PMC3297424
Related URLs:
URI: http://repository.cshl.edu/id/eprint/27025

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