Transcription of a nematode trans-spliced leader RNA require internal elements for both initiation and 3' end-formation

Hannon, G. J., Maroney, P. A., Ayers, D. G., Shambaugh, J. D., Nilsen, T. W. (1990) Transcription of a nematode trans-spliced leader RNA require internal elements for both initiation and 3' end-formation. EMBO Journal, 9 (6). pp. 1915-1921. ISSN 02614189 (ISSN)

URL: http://www.ncbi.nlm.nih.gov/pubmed/2347310

Abstract

We have used block substitution mutagenesis and in vitro transcription to define sequence elements important for efficient initiation and 3' end-formation to the trans-spliced leader RNA (SL RNA) of the parasitic nematode Ascaris lumbricoides. These experiments indicate that the SL RNA has an unusual promoter structure containing elements which include the 22 nt trans-spliced leader exon itself. Efficient transcription is correlated with the binding of a factor to the 22 nt (SL) sequence mutations within the SL which abolish transcription lead to a loss in binding of this factor. In addition to internal sequences, synthesis of SL RNA in vitro requires an element centered 50 bases upstream of the cap site. Mutations within this element dramatically affect the level of SL RNA synthesis but do not affect accuracy of initiation. Finally, all of the information required for accurate 3' end-formation of SL RNA lies within the transcribed region. Thus, the arrangement of sequences necessary for the synthesis of SL RNAs does not resemble that of sequences important for the synthesis of vertebrate U snRNAs despite the similarities between SL RNAs and U snRNAs.

Item Type: Paper
Uncontrolled Keywords: Ascaris lumbricoides RNA polymerase II spliced leader RNA transcription rna polymerase animal cell article nematode nonhuman priority journal rna gene rna splicing Animal Ascaris Base Sequence Deoxyribonuclease I In Vitro Molecular Sequence Data Mutation Restriction Mapping RNA, Messenger Support, Non-U.S. Gov't Support, U.S. Gov't, Non-P.H.S. Support, U.S. Gov't, P.H.S. Transcription, Genetic Animalia Vertebrata
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNA regulation
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNA splicing
CSHL Authors:
Communities: CSHL labs > Hannon lab
Depositing User: Matt Covey
Date: 1990
Date Deposited: 09 Jan 2013 17:34
Last Modified: 09 Jan 2013 17:34
PMCID: PMC551899
Related URLs:
URI: http://repository.cshl.edu/id/eprint/26503

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