Intramolecular base pairing between the nematode spliced leader and its 5' splice site is not essential for trans-splicing in vitro

Maroney, P. A., Hannon, G. J., Shambaugh, J. D., Nilsen, T. W. (1991) Intramolecular base pairing between the nematode spliced leader and its 5' splice site is not essential for trans-splicing in vitro. EMBO Journal, 10 (12). pp. 3869-3875. ISSN 02614189 (ISSN)

URL: http://www.ncbi.nlm.nih.gov/pubmed/1935906

Abstract

The spliced leader RNAs of both trypanosomes and nematodes can form similar secondary structures where the trans-splice donor site is involved in intramolecular base pairing with the spliced leader sequence. It has been proposed that this base pairing could serve to activate autonomously the SL RNA splice donor site. Here, we have examined exon requirements for trans-splicing in a nematode cell free system. Complete disruption of secondary structure interactions at and around the trans-splice donor site did not affect the ability of the SL RNA to function in trans-splicing. In addition, the highly conserved 22 nt sequence could be productively replaced by artificial exons ranging in size from 2 to 246 nucleotides. These results reinforce the view that the 'intron' portion of the SL RNA functions as an independent Sm snRNP whose role is to deliver exon sequences to the trans-spliceosome.

Item Type: Paper
Uncontrolled Keywords: Ascaris lumbricoides Nematode RNA processing Sm snRNP Trans-splicing base rna signal peptide article base pairing binding site cell free system donor site exon nonhuman nucleic acid base substitution priority journal rna sequence rna splicing site directed mutagenesis trypanosoma Animal Base Composition Base Sequence Exons Molecular Sequence Data Mutagenesis, Site-Directed Mutation Nematoda Nucleic Acid Conformation Protein Sorting Signals RNA, Messenger Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S.
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNA splicing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > splicing factor
CSHL Authors:
Communities: CSHL labs > Hannon lab
Depositing User: Matt Covey
Date: 1991
Date Deposited: 09 Jan 2013 17:36
Last Modified: 09 Jan 2013 17:36
PMCID: PMC453124
Related URLs:
URI: http://repository.cshl.edu/id/eprint/26498

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