Radiation-induced cell cycle arrest compromised by p21 deficiency

Bruarolas, J., Chandrasekaran, C., Gordon, J. I., Beach, D., Jacks, T., Hannon, G. J. (1995) Radiation-induced cell cycle arrest compromised by p21 deficiency. Nature, 377 (6549). pp. 552-557. ISSN 00280836 (ISSN)

URL: http://www.ncbi.nlm.nih.gov/pubmed/7566157
DOI: 10.1038/377552a0

Abstract

THE protein p21 is a dual inhibitor of cyclin-dependent kinases and proliferating-cell nuclear antigen (PCNA), both of which are required for passage through the cell cycle. The p21 gene is under the transcriptional control of p53, suggesting that p21 might promote p53-dependent cell cycle arrest or apoptosis. p21 has also been implicated in cell senescence and in cell-cycle withdrawal upon terminal differentiation. Here we investigate the role of p21 in these processes using chimaeric mice composed partly of p21(-/-) and partly of p21(+/+) cells. Immunohistochemical studies of the p21(+/+) and p21(-/-) components of adult small intestine indicated that deletion of p21 had no detectable effect on the migration-associated differentiation of the four principal intestinal epithelial cell lineages or on p53-dependent apoptosis following irradiation. However, p21(-/-) embryo fibroblasts are impaired in their ability to undergo G1 arrest following DNA damage.

Item Type: Paper
Uncontrolled Keywords: cyclin dependent kinase cycline protein p21 protein p53 animal cell apoptosis article cell cycle g1 phase chimera controlled study dna damage embryo fibroblast immunohistochemistry intestine epithelium cell mouse nonhuman priority journal protein deficiency radiation mutagenesis senescence transcription regulation Animal Cell Cycle Cell Differentiation Cell Division Cell Line Cells, Cultured Cyclin-Dependent Kinases Cyclins Enzyme Inhibitors Gamma Rays Gene Targeting Intestine, Small Mice Mice, Inbred C57BL Proliferating Cell Nuclear Antigen Protein-Serine-Threonine Kinases Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S.
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > p21
CSHL Authors:
Communities: CSHL labs > Hannon lab
Depositing User: Matt Covey
Date: 1995
Date Deposited: 09 Jan 2013 16:59
Last Modified: 09 Jan 2013 16:59
Related URLs:
URI: http://repository.cshl.edu/id/eprint/26485

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