siRNAs and shRNAs: Skeleton keys to the human genome

Paddison, P. J., Hannon, G. J. (2003) siRNAs and shRNAs: Skeleton keys to the human genome. Current Opinion in Molecular Therapeutics, 5 (3). pp. 217-224. ISSN 14648431 (ISSN)

URL: http://www.ncbi.nlm.nih.gov/pubmed/12870430

Abstract

The use of RNA interference (RNAi) to evoke gene silencing in mammalian cell systems is rapidly becoming a standard laboratory technique. Recent experiments have also proven the utility of RNAi in animals. The next few years will bear witness to the first RNAi-based genome-wide screens in mammals, breaking the barrier to the use of mammalian cells as a genetic system. In this review, some of the key features of RNAi in mammalian systems are discussed.

Item Type: Paper
Uncontrolled Keywords: Functional genomics Gene silencing Human disease Mouse models RNAi shRNA siRNA double stranded RNA messenger RNA nuclease ribonuclease III RNA RNA helicase gene expression regulation gene targeting genetic screening genome analysis genomics human human genome laboratory test mammal cell nonhuman review RNA analysis RNA structure RNA translation standard Animals Genome, Human Humans Nucleic Acid Conformation RNA Interference RNA, Double-Stranded RNA, Small Interfering Animalia Mammalia
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > genomes
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > shRNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > siRNA
CSHL Authors:
Communities: CSHL labs > Hannon lab
Watson School > Publications
Depositing User: Matt Covey
Date: 2003
Date Deposited: 09 Jan 2013 16:51
Last Modified: 19 Sep 2014 14:44
URI: http://repository.cshl.edu/id/eprint/26457

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