Silva, J. M., Sachidanandam, R., Hannon, G. J. (2003) Free energy lights the path toward more effective RNAi. Nature Genetics, 35 (4). pp. 303-305. ISSN 10614036 (ISSN)
DOI: 10.1038/ng1203-303
Abstract
Silencing gene expression by RNA interference (RNAi) has become a daily activity for mammalian cell biologists. Those who often use this approach have come to appreciate its occasional frustrations and potential limitations. A pair of recent papers in Cell provides rules for designing short interfering RNAs (siRNAs) that are both validated at the practical level and understood mechanistically.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | adenosine triphosphate cell extract double stranded RNA RNA helicase Drosophila melanogaster gene sequence gene silencing gene targeting genetic transfection nonhuman priority journal protein degradation short survey Animals Drug Design Energy Transfer Humans RNA Interference RNA Stability RNA, Small Interfering Mammalia Melanogaster |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNAi |
| CSHL Authors: | |
| Communities: | CSHL labs > Hannon lab |
| Depositing User: | Matt Covey |
| Date: | 2003 |
| Date Deposited: | 09 Jan 2013 16:52 |
| Last Modified: | 09 Jan 2013 16:52 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/26455 |
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