Analysis of large-scale sequencing of small RNAs

Olson, A. J., Brennecke, J., Aravin, A. A., Hannon, G. J., Sachidanandam, R. (2008) Analysis of large-scale sequencing of small RNAs. Pacific Symposium on Biocomputing. pp. 126-136. ISSN 17935091 (ISSN)

[img]
Preview
PDF (Paper)
Hannon PacSymBioComp 2008.pdf - Published Version

Download (1049Kb) | Preview
URL: http://www.ncbi.nlm.nih.gov/pubmed/18229681

Abstract

The advent of large-scale sequencing has opened up new areas of research, such as the study of Piwi-interacting small RNAs (piRNAs). piRNAs are longer than miRNAs, close to 30 nucleotides in length, involved in various functions, such as the suppression of transposons in germline. Since a large number of them (many tens of thousands) are generated from a wide range of positions in the genome, large-scale sequencing is the only way to study them. The key to understanding their genesis and biological roles is efficient analysis, which is complicated by the large volumes of sequence data. Taking account of the underlying biology is also important. We describe here novel analyses techniques and tools applied to small RNAs from germ cells in D. melanogaster, that allowed us to infer mechanism and biological function.

Item Type: Paper
Uncontrolled Keywords: AGO3 protein, Drosophila aubergine protein, Drosophila Drosophila protein initiation factor RNA small interfering RNA unclassified drug animal article biology Drosophila melanogaster genetics genome germ cell multigene family sequence analysis statistics Animals Computational Biology Drosophila Proteins Genome, Insect Germ Cells Peptide Initiation Factors RNA, Small Interfering Sequence Analysis, RNA
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
Investigative techniques and equipment
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > piRNA
Investigative techniques and equipment > whole exome sequencing
Investigative techniques and equipment > assays > whole exome sequencing
CSHL Authors:
Communities: CSHL labs > Hannon lab
Depositing User: Matt Covey
Date: 2008
Date Deposited: 09 Jan 2013 16:54
Last Modified: 09 Jan 2013 16:54
Related URLs:
URI: http://repository.cshl.edu/id/eprint/26447

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving