Comparative Analysis of Argonaute-Dependent Small RNA Pathways in Drosophila

Zhou, R., Hotta, I., Denli, A. M., Hong, P., Perrimon, N., Hannon, G. J. (2008) Comparative Analysis of Argonaute-Dependent Small RNA Pathways in Drosophila. Molecular Cell, 32 (4). pp. 592-599. ISSN 10972765 (ISSN)

URL: http://www.ncbi.nlm.nih.gov/pubmed/19026789
DOI: 10.1016/j.molcel.2008.10.018

Abstract

The specificity of RNAi pathways is determined by several classes of small RNAs, which include siRNAs, piRNAs, endo-siRNAs, and microRNAs (miRNAs). These small RNAs are invariably incorporated into large Argonaute (Ago)-containing effector complexes known as RNA-induced silencing complexes (RISCs), which they guide to silencing targets. Both genetic and biochemical strategies have yielded conserved molecular components of small RNA biogenesis and effector machineries. However, given the complexity of these pathways, there are likely to be additional components and regulators that remain to be uncovered. We have undertaken a comparative and comprehensive RNAi screen to identify genes that impact three major Ago-dependent small RNA pathways that operate in Drosophila S2 cells. We identify subsets of candidates that act positively or negatively in siRNA, endo-siRNA, and miRNA pathways. Our studies indicate that many components are shared among all three Argonaute-dependent silencing pathways, though each is also impacted by discrete sets of genes. © 2008 Elsevier Inc. All rights reserved.

Item Type: Paper
Uncontrolled Keywords: PROTEINS RNA argonaute protein microRNA RNA induced silencing complex small interfering RNA animal cell article biogenesis comparative gene mapping comparative study Drosophila genetic identification nonhuman RNA interference Animals Cell Line Drosophila melanogaster Drosophila Proteins Gene Silencing Genes, Insect MicroRNAs Models, Biological RNA, Small Interfering RNA, Untranslated RNA-Induced Silencing Complex
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
organism description > animal > insect > Drosophila
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > argonaute proteins
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > sRNA
CSHL Authors:
Communities: CSHL labs > Hannon lab
Watson School > Publications
Depositing User: Matt Covey
Date: 2008
Date Deposited: 09 Jan 2013 16:42
Last Modified: 22 Sep 2014 16:23
PMCID: PMC2615197
Related URLs:
URI: http://repository.cshl.edu/id/eprint/26443

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