Cell contact-dependent acquisition of cellular and viral nonautonomously encoded small RNAs

Rechavi, O., Erlich, Y., Amram, H., Flomenblit, L., Karginov, F. V., Goldstein, I., Hannon, G. J., Kloog, Y. (2009) Cell contact-dependent acquisition of cellular and viral nonautonomously encoded small RNAs. Genes and Development, 23 (16). pp. 1971-1979. ISSN 08909369 (ISSN)

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URL: http://www.ncbi.nlm.nih.gov/pubmed/19684116
DOI: 10.1101/gad.1789609

Abstract

In some organisms, small RNA pathways can act nonautonomously, with responses spreading from cell to cell. Dedicated intercellular RNA delivery pathways have not yet been characterized in mammals, although secretory compartments have been found to contain RNA. Here we show that, upon cell contact, T cells acquire from B cells small RNAs that can impact the expression of target genes in the recipient T cells. Synthetic microRNA (miRNA) mimetics, viral miRNAs expressed by infected B cells, and endogenous miRNAs could all be transferred into T cells. These mechanisms may allow small RNA-mediated communication between immune cells. The documented transfer of viral miRNAs raises the possible exploitation of these pathways for viral manipulation of the host immune response. © 2009 by Cold Spring Harbor Laboratory Press.

Item Type: Paper
Uncontrolled Keywords: Immunity Intercellular transfer microRNA shRNA virus RNA article B lymphocyte cell communication cell contact controlled study gene expression gene targeting human human cell immune response immunocompetent cell priority journal RNA transport signal transduction T lymphocyte cell culture gene expression regulation leukemia cell line metabolism Mammalia B-Lymphocytes Cells, Cultured Humans Jurkat Cells MicroRNAs RNA, Viral T-Lymphocytes
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > sRNA
CSHL Authors:
Communities: CSHL Post Doctoral Fellows
CSHL labs > Hannon lab
CSHL Cancer Center Shared Resources > Animal Services
CSHL Cancer Center Shared Resources > Bioinformatics Service
CSHL Cancer Center Shared Resources > Flow Cytometry Service
Watson School > Publications
Depositing User: Matt Covey
Date: 2009
Date Deposited: 09 Jan 2013 16:43
Last Modified: 30 Dec 2014 16:52
PMCID: PMC2725935
Related URLs:
URI: http://repository.cshl.edu/id/eprint/26437

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