Processing of Drosophila endo-siRNAs depends on a specific Loquacious isoform

Zhou, R., Czech, B., Brennecke, J., Sachidanandam, R., Wohlschlegel, J. A., Perrimon, N., Hannon, G. J. (2009) Processing of Drosophila endo-siRNAs depends on a specific Loquacious isoform. RNA, 15 (10). pp. 1886-1895. ISSN 13558382 (ISSN)

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URL: http://www.ncbi.nlm.nih.gov/pubmed/19635780
DOI: 10.1261/rna.1611309

Abstract

Drosophila melanogaster expresses three classes of small RNAs, which are classified according to their mechanisms of biogenesis. MicroRNAs are ∼22-23 nucleotides (nt), ubiquitously expressed small RNAs that are sequentially processed from hairpin-like precursors by Drosha/Pasha and Dcr-1/Loquacious complexes. MicroRNAs usually associate with AGO1 and regulate the expression of protein-coding genes. Piwi-interacting RNAs (piRNAs) of ∼24-28 nt associate with Piwi-family proteins and can arise from single-stranded precursors. piRNAs function in transposon silencing and are mainly restricted to gonadal tissues. Endo-siRNAs are found in both germline and somatic tissues. These ∼21-nt RNAs are produced by a distinct Dicer, Dcr-2, and do not depend on Drosha/Pasha complexes. They predominantly bind to AGO2 and target both mobile elements and protein-coding genes. Surprisingly, a subset of endo-siRNAs strongly depend for their production on the dsRNA-binding protein Loquacious (Loqs), thought generally to be a partner for Dcr-1 and a cofactor for miRNA biogenesis. Endo-siRNA production depends on a specific Loqs isoform, Loqs-PD, which is distinct from the one, Loqs-PB, required for the production of microRNAs. Paralleling their roles in the biogenesis of distinct small RNA classes, Loqs-PD and Loqs-PB bind to different Dicer proteins, with Dcr-1/Loqs-PB complexes and Dcr-2/Loqs-PD complexes driving microRNA and endo-siRNA biogenesis, respectively. Copyright © 2009 RNA Society.

Item Type: Paper
Uncontrolled Keywords: Dicer Double-stranded RNA-binding proteins (dsRBPs) Drosophila melanogaster Endo-siRNA processing Loquacious Transposon silencing argonaute 2 protein binding protein double stranded RNA microRNA nucleotide Piwi interacting RNA small interfering RNA unclassified drug article biogenesis Drosophila gene expression gene silencing genetic regulation germline micronucleus insect cell nonhuman priority journal protein expression protein function proteomics RNA binding RNA processing transposon Animals DNA Transposable Elements Germ Cells RNA, Small Interfering
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
organism description > animal > insect > Drosophila
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > siRNA
CSHL Authors:
Communities: CSHL labs > Hannon lab
Depositing User: Matt Covey
Date: 2009
Date Deposited: 09 Jan 2013 16:13
Last Modified: 09 Nov 2017 21:28
PMCID: PMC2743050
Related URLs:
URI: http://repository.cshl.edu/id/eprint/26432

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