A genome-scale shRNA resource for transgenic RNAi in Drosophila

Ni, J. Q., Zhou, R., Czech, B., Liu, L. P., Holderbaum, L., Yang-Zhou, D., Shim, H. S., Tao, R., Handler, D., Karpowicz, P., Binari, R., Booker, M., Brennecke, J., Perkins, L. A., Hannon, G. J., Perrimon, N. (2011) A genome-scale shRNA resource for transgenic RNAi in Drosophila. Nature Methods, 8 (5). pp. 405-407. ISSN 15487091 (ISSN)

URL: http://www.ncbi.nlm.nih.gov/pubmed/21460824
DOI: 10.1038/nmeth.1592

Abstract

Existing transgenic RNAi resources in Drosophila melanogaster based on long double-stranded hairpin RNAs are powerful tools for functional studies, but they are ineffective in gene knockdown during oogenesis, an important model system for the study of many biological questions. We show that shRNAs, modeled on an endogenous microRNA, are extremely effective at silencing gene expression during oogenesis. We also describe our progress toward building a genome-wide shRNA resource. © 2011 Nature America, Inc. All rights reserved.

Item Type: Paper
Uncontrolled Keywords: microRNA short hairpin RNA article Drosophila gene expression gene silencing nonhuman oocyte development priority journal RNA interference Animals Animals, Genetically Modified Base Sequence DNA Primers Drosophila melanogaster Female Gene Knockdown Techniques Genetic Techniques Genetic Vectors Genome, Insect MicroRNAs Oogenesis RNA, Small Interfering
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
organism description > animal > insect > Drosophila
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics > design > nucleic acid design
bioinformatics > genomics and proteomics > design > nucleic acid design > RNAI Design
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNAi
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > shRNA
CSHL Authors:
Communities: CSHL labs > Hannon lab
CSHL Cancer Center Shared Resources > Bioinformatics Service
CSHL Cancer Center Shared Resources > DNA Sequencing Service
Depositing User: Matt Covey
Date: 2011
Date Deposited: 09 Jan 2013 16:15
Last Modified: 26 Dec 2014 19:58
PMCID: PMC3489273
Related URLs:
URI: http://repository.cshl.edu/id/eprint/26428

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