Ordered progression of stage-specific miRNA profiles in the mouse B2 B-cell lineage

Spierings, D. C., McGoldrick, D., Hamilton-Easton, A. M., Neale, G., Murchison, E. P., Hannon, G. J., Green, D. R., Withoff, S. (2011) Ordered progression of stage-specific miRNA profiles in the mouse B2 B-cell lineage. Blood, 117 (20). pp. 5340-5349. ISSN 00064971 (ISSN)

URL: http://www.ncbi.nlm.nih.gov/pubmed/21403133
DOI: 10.1182/blood-2010-10-316034

Abstract

Micro-RNAs (miRNAs) have been recognized as critical regulators of gene expression, and deregulation of miRNA expression has been implicated in a wide spectrumofdiseases. To provide aframework for the role of miRNAs in B-cell development and malignancy, we deepsequenced miRNAs from B1 cells and 10 developmental stages that can be identified within the mouse B2 B-cell lineage. The expression profiles of the 232 known miRNAs that are expressed during B-cell development display stage-specific induction patterns, yet hierarchical clustering analysis showed relationships that are in full agreement with the model of the B2 B-cell developmental pathway. Analysis of exemplary miRNA expression profiles (miR-150, miR-146a, miR-155, miR-181) confirmed that our data are in agreement with previous results. The high resolution of the expression data allowed for the identification of the sequential expression of oncomir-1/miR-17-92 and its paralogs miR-106a-363 and miR-106b-25 in subsequent developmental stages in the BM. Further, we have identified and validated 45 novel miRNAs and 6 novel miRNA candidates expressed in developing B cells. © 2011 by The American Society of Hematology.

Item Type: Paper
Uncontrolled Keywords: microRNA microRNA 106a microRNA 106b microRNA 146a microRNA 150 microRNA 155 microRNA 181 unclassified drug animal cell article B lymphocyte cell maturation deregulation developmental stage disease course gene expression mouse nonhuman priority journal protein expression Animals B-Lymphocyte Subsets Cell Differentiation Cell Lineage Cell Transformation, Neoplastic Gene Expression Profiling Gene Expression Regulation, Developmental Mice Mice, Inbred C57BL MicroRNAs RNA, Untranslated
Subjects: organs, tissues, organelles, cell types and functions > cell types and functions > cell types > B cells
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > B cells
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > B cells

bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > miRNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > miRNA

organism description > animal > mammal > rodent > mouse
CSHL Authors:
Communities: CSHL Cancer Center Program > Cancer Genetics
CSHL Cancer Center Shared Resources > Bioinformatics Service
CSHL Cancer Center Shared Resources > DNA Sequencing Service
CSHL Cancer Center Shared Resources > Flow Cytometry Service
CSHL labs > Hannon lab
Watson School > Publications
Depositing User: Matt Covey
Date: 2011
Date Deposited: 09 Jan 2013 16:17
Last Modified: 03 Jan 2019 21:37
PMCID: PMC3109709
Related URLs:
URI: http://repository.cshl.edu/id/eprint/26426

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