Vreteno, a gonad-specific protein, is essential for germline development and primary pirna biogenesis in Drosophila

Zamparini, A. L., Davis, M. Y., Malone, C. D., Vieira, E., Zavadil, J., Sachidanandam, R., Hannon, G. J., Lehmann, R. (2011) Vreteno, a gonad-specific protein, is essential for germline development and primary pirna biogenesis in Drosophila. Development, 138 (18). pp. 4039-4050. ISSN 09501991 (ISSN)

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URL: http://www.ncbi.nlm.nih.gov/pubmed/21831924
DOI: 10.1242/dev.069187

Abstract

In Drosophila, Piwi proteins associate with Piwi-interacting RNAs (piRNAs) and protect the germline genome by silencing mobile genetic elements. This defense system acts in germline and gonadal somatic tissue to preserve germline development. Genetic control for these silencing pathways varies greatly between tissues of the gonad. Here, we identified Vreteno (Vret), a novel gonad-specific protein essential for germline development. Vret is required for piRNA-based transposon regulation in both germline and somatic gonadal tissues. We show that Vret, which contains Tudor domains, associates physically with Piwi and Aubergine (Aub), stabilizing these proteins via a gonad-specific mechanism that is absent in other fly tissues. In the absence of vret, Piwi-bound piRNAs are lost without changes in piRNA precursor transcript production, supporting a role for Vret in primary piRNA biogenesis. In the germline, piRNAs can engage in an Aub- and Argonaute 3 (AGO3)-dependent amplification in the absence of Vret, suggesting that Vret function can distinguish between primary piRNAs loaded into Piwi-Aub complexes and piRNAs engaged in the amplification cycle. We propose that Vret plays an essential role in transposon regulation at an early stage of primary piRNA processing. © 2011. Published by The Company of Biologists Ltd.

Item Type: Paper
Uncontrolled Keywords: Aubergine Drosophila Germline stem cell piRNAs Piwi Soma Transposon Tudor Piwi interacting RNA protein protein vreteno unclassified drug article biogenesis germ line gonad development nonhuman nucleotide sequence priority journal protein analysis protein domain protein function protein protein interaction RNA analysis RNA processing RNA synthesis Animals Animals, Genetically Modified Drosophila melanogaster Drosophila Proteins Female Gene Expression Profiling Gene Expression Regulation, Developmental Gene Silencing Germ Cells Gonads Male Microarray Analysis Organ Specificity Ovary Protein Structure, Tertiary RNA Processing, Post-Transcriptional RNA, Small Interfering Solanum melongena
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
organism description > animal > insect > Drosophila
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > piRNA
CSHL Authors:
Communities: CSHL labs > Hannon lab
CSHL labs > Sachidanandam lab
Watson School > Publications
CSHL Cancer Center Shared Resources > Bioinformatics Service
CSHL Cancer Center Shared Resources > DNA Sequencing Service
CSHL Cancer Center Shared Resources > Microscopy Service
Depositing User: Matt Covey
Date: 2011
Date Deposited: 09 Jan 2013 16:18
Last Modified: 26 Dec 2014 19:30
PMCID: PMC3160098
Related URLs:
URI: http://repository.cshl.edu/id/eprint/26423

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