Confirming specificity of RNAi in mammalian cells

Lassus, P., Rodriguez, J., Lazebnik, Y. (August 2002) Confirming specificity of RNAi in mammalian cells. Sci STKE, 2002 (147). pl13. ISSN 1525-8882 (Electronic)1525-8882 (Linking)

URL: http://stke.sciencemag.org/cgi/content/abstract/si...
DOI: 10.1126/stke.2002.147.pl13

Abstract

RNA interference (RNAi) is a process of sequence-specific gene silencing. Recent advances in the understanding of RNAi have provided practical tools to silence gene expression in mammalian cells, opening new possibilities for studying the functions of genes and proteins. It is important to ensure that an observed effect of RNAi is due to silencing of the intended target. Indeed, it is possible that an siRNA may silence more than one messenger RNA that is homologous in the region complementary to the siRNA. Considering that we know little about how RNAi works in mammalian cells, other artifacts may be yet to be recognized. Thus, we suggest approaches to rescue the effect of RNAi by ectopically expressing the protein of interest. These approaches involve introducing silent mutations into the complementary DNA of the protein and targeting RNAi to the untranslated regions of the gene.

Item Type: Paper
Additional Information: Lassus, Patrice Rodriguez, Joe Lazebnik, Yuri United States Science's STKE : signal transduction knowledge environment Sci STKE. 2002 Aug 27;2002(147):pl13.
Uncontrolled Keywords: 3' Untranslated Regions/genetics/metabolism Adenoviridae Animals Cell Line Cell Line, Transformed/virology Citrate (si)-Synthase/genetics Fibroblasts/chemistry/enzymology/metabolism/virology Gene Silencing/physiology Humans Mutagenesis, Site-Directed Oligoribonucleotides/chemical synthesis RNA Processing, Post-Transcriptional/genetics/physiology RNA, Double-Stranded/genetics/metabolism RNA, Messenger/genetics/metabolism RNA, Small Interfering RNA, Untranslated/ genetics/ metabolism Transfection/instrumentation/methods
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > design
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
bioinformatics > genomics and proteomics > design > nucleic acid design
bioinformatics > genomics and proteomics > design > nucleic acid design > RNAI Design
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNAi
CSHL Authors:
Communities: CSHL labs > Labeznik lab
Depositing User: Matt Covey
Date: 27 August 2002
Date Deposited: 12 Dec 2012 17:29
Last Modified: 12 Dec 2012 17:29
URI: http://repository.cshl.edu/id/eprint/26382

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