Immunofluorescence localization of nuclear proteins

Spector, David L. (2011) Immunofluorescence localization of nuclear proteins. Cold Spring Harbor Protocols, 2011 (10). pp. 1276-1280. ISSN 1559-6095

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URL: http://www.ncbi.nlm.nih.gov/pubmed/21969615
DOI: 10.1101/pdb.prot065755

Abstract

Many nuclear proteins have been successfully localized using immunofluorescence microscopy. These proteins span all nuclear domains, including the nuclear envelope, nuclear lamina, nucleolus, chromatin-associated proteins, and proteins associated with RNA metabolism and nuclear bodies. This article describes a general method for localizing nuclear proteins. Cells grown on coverslips are fixed in either formaldehyde or methanol and permeabilized in Triton X-100. Incubating the cells with primary antibody and fluorescently conjugated secondary antibody allows visualization of the target antigen.

Item Type: Paper
Uncontrolled Keywords: Cells, Cultured Fluorescent Antibody Technique Formaldehyde Methanol Nuclear Proteins Octoxynol Surface-Active Agents
Subjects: Investigative techniques and equipment
Investigative techniques and equipment > microscopy > flourescence microscopy
Investigative techniques and equipment > microscopy > immunoflourescence microscopy
Investigative techniques and equipment > microscopy
CSHL Authors:
Communities: CSHL labs > Spector lab
Depositing User: Matt Covey
Date Deposited: 10 Dec 2012 20:02
Last Modified: 29 Jan 2015 21:12
Related URLs:
URI: http://repository.cshl.edu/id/eprint/26373

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