A genetic locus targeted to the nuclear periphery in living cells maintains its transcriptional competence

Kumaran, R. I., Spector, D. L. (2008) A genetic locus targeted to the nuclear periphery in living cells maintains its transcriptional competence. Journal of Cell Biology, 180. pp. 51-65. ISSN 0021-9525

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URL: http://www.ncbi.nlm.nih.gov/pubmed/18195101
DOI: 10.1083/jcb.200706060

Abstract

The peripheral nuclear lamina, which is largely but not entirely associated with inactive chromatin, is considered to be an important determinant of nuclear structure and gene expression. We present here an inducible system to target a genetic locus to the nuclear lamina in living mammalian cells. Using three-dimensional time-lapse microscopy, we determined that targeting of the locus requires passage through mitosis. Once targeted, the locus remains anchored to the nuclear periphery in interphase as well as in daughter cells after passage through a subsequent mitosis. Upon transcriptional induction, components of the gene expression machinery are recruited to the targeted locus, and we visualized nascent transcripts at the nuclear periphery. The kinetics of transcriptional induction at the nuclear lamina is similar to that observed at an internal nuclear region. This new cell system provides a powerful approach to study the dynamics of gene function at the nuclear periphery in living cells.

Item Type: Paper
Uncontrolled Keywords: Cell Line Chromosomes Gene Expression Regulation Humans Interphase Kinetics Lamins Luminescent Proteins Mitosis Models, Genetic Nuclear Lamina Transcription, Genetic
Subjects: bioinformatics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
CSHL Authors:
Communities: CSHL labs > Spector lab
Depositing User: Matt Covey
Date Deposited: 10 Dec 2012 20:02
Last Modified: 28 Jan 2015 21:02
PMCID: PMC2213611
Related URLs:
URI: http://repository.cshl.edu/id/eprint/26361

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