Sequential entry of components of the gene expression machinery into daughter nuclei

Prasanth, K. V. , Sacco-Bubulya, P. A., Prasanth, S. G., Spector, D. L. (2003) Sequential entry of components of the gene expression machinery into daughter nuclei. Molecular Biology of the Cell, 14 (3). pp. 1043-1057. ISSN 1059-1524

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URL: http://www.ncbi.nlm.nih.gov/pubmed/12631722
DOI: 10.1091/mbc.E02-10-0669

Abstract

In eukaryotic cells, RNA polymerase II (RNA pol II) transcription and pre-mRNA processing are coordinated events. We have addressed how individual components of the transcription and pre-mRNA processing machinery are organized during mitosis and subsequently recruited into the newly formed daughter nuclei. Interestingly, localization studies of numerous RNA pol II transcription and pre-mRNA processing factors revealed a nonrandom and sequential entry of these factors into daughter nuclei after nuclear envelope/lamina formation. The initiation competent form of RNA pol II and general transcription factors appeared in the daughter nuclei simultaneously, but prior to pre-mRNA processing factors, whereas the elongation competent form of RNA pol II was detected even later. The differential entry of these factors rules out the possibility that they are transported as a unitary complex. Telophase nuclei were competent for transcription and pre-mRNA splicing concomitant with the initial entry of the respective factors. In addition, our results revealed a low turnover rate of transcription and pre-mRNA splicing factors during mitosis. We provide evidence to support a model in which the entry of the RNA pol II gene expression machinery into newly forming daughter nuclei is a staged and ordered process.

Item Type: Paper
Uncontrolled Keywords: Cell Nucleus Gene Expression Regulation HeLa Cells Humans Mitosis RNA Polymerase II RNA Precursors RNA Processing, Post-Transcriptional RNA Splicing RNA, Messenger Transcription Factors Transcription, Genetic
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
CSHL Authors:
Communities: CSHL labs > Spector lab
Depositing User: Matt Covey
Date Deposited: 10 Dec 2012 20:01
Last Modified: 29 Jan 2015 16:08
PMCID: PMC151578
Related URLs:
URI: http://repository.cshl.edu/id/eprint/26356

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