Metabolic-energy-dependent movement of PML bodies within the mammalian cell nucleus

Muratani, M., Gerlich, D., Janicki, S. M., Gebhard, M., Eils, R., Spector, D. L. (2002) Metabolic-energy-dependent movement of PML bodies within the mammalian cell nucleus. Nature Cell Biology, 4. pp. 106-110. ISSN 1465-7392

URL: http://www.ncbi.nlm.nih.gov/pubmed/11753375
DOI: 10.1038/ncb740

Abstract

Promyelocytic leukaemia (PML) nuclear bodies are present in most mammalian cell nuclei. PML bodies are disrupted by PML retinoic acid receptor alpha (RAR alpha) oncoproteins in acute promyelocytic leukaemia. These bodies contain numerous proteins, including Sp100, SUMO-1, HAUSP(USP7), CBP and BLM, and they have been implicated in aspects of transcriptional regulation or as nuclear storage depots. Here, we show that three classes of PML nuclear bodies can be distinguished, on the basis of their dynamic properties in living cells. One class of PML bodies is particularly noteworthy in that it moves by a metabolic-energy-dependent mechanism. This represents the first example of metabolic-energy-dependent transport of a nuclear body within the mammalian cell nucleus.

Item Type: Paper
Uncontrolled Keywords: Animals Antigens, Nuclear Autoantigens Bacterial Proteins Cell Line Cell Nucleus Cricetinae Energy Metabolism Humans Leukemia, Promyelocytic, Acute Luminescent Proteins Microscopy, Fluorescence Models, Biological Movement Myosins Neoplasm Proteins Nuclear Proteins Oncogene Proteins, Fusion Recombinant Fusion Proteins Time Factors Transcription Factors Tumor Suppressor Proteins
Subjects: diseases & disorders > cancer
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription
bioinformatics > genomics and proteomics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein expression
organs, tissues, organelles, cell types and functions > tissues types and functions > transport > protein transport
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > transcription factor
CSHL Authors:
Communities: CSHL labs > Spector lab
Watson School > Publications
Depositing User: Matt Covey
Date Deposited: 10 Dec 2012 19:57
Last Modified: 29 Jan 2015 16:36
Related URLs:
URI: http://repository.cshl.edu/id/eprint/26352

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