Methylation of histone H3 at Lys-9 is an early mark on the X chromosome during X inactivation

Heard, E., Rougeulle, C., Arnaud, D., Avner, P., Allis, C. D., Spector, D. L. (2001) Methylation of histone H3 at Lys-9 is an early mark on the X chromosome during X inactivation. Cell, 107 (6). pp. 727-738. ISSN 0092-8674

URL: http://www.ncbi.nlm.nih.gov/pubmed/11747809
DOI: 10.1016/S0092-8674(01)00598-0

Abstract

Coating of the X chromosome by Xist RNA is an essential trigger for X inactivation. However, little is known about the early chromatin remodeling events that transform this signal into transcriptional silencing. Here we report that methylation of histone H3 lysine 9 on the inactive X chromosome occurs immediately after Xist RNA coating and before transcriptional inactivation of X-linked genes. X-chromosomal H3 Lys-9 methylation occurs during the same window of time as H3 Lys-9 hypoacetylation and H3 Lys-4 hypomethylation. Histone H3 modifications thus represent the earliest known chromatin changes during X inactivation. We also identify a unique "hotspot" of H3 Lys-9 methylation 5' to Xist, and we propose that this acts as a nucleation center for Xist RNA-dependent spread of inactivation along the X chromosome via H3 Lys-9 methylation.

Item Type: Paper
Uncontrolled Keywords: A Kinase Anchor Proteins Acetylation Adaptor Proteins, Signal Transducing Animals Cell Cycle Proteins Cell Differentiation Cells, Cultured Chromatin Chromosomal Proteins, Non-Histone Cyclin-Dependent Kinase Inhibitor p27 DNA-Binding Proteins Dosage Compensation, Genetic Enzyme Inhibitors Female Fibroblasts Gene Silencing Histones In Situ Hybridization, Fluorescence Male Methyl-CpG-Binding Protein 2 Methylation Mice Models, Biological Oncogene Proteins Proto-Oncogene Proteins RNA RNA, Untranslated Repressor Proteins Stem Cells Transcription Factors Tumor Suppressor Proteins X Chromosome
Subjects: bioinformatics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosome
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function > chromosome

bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein methylation > histone methylation
CSHL Authors:
Communities: CSHL labs > Spector lab
Depositing User: Matt Covey
Date Deposited: 10 Dec 2012 19:57
Last Modified: 29 Jan 2015 16:46
Related URLs:
URI: http://repository.cshl.edu/id/eprint/26350

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