RNA polymerase II targets pre-mRNA splicing factors to transcription sites in vivo

Misteli, T., Spector, D. L. (1999) RNA polymerase II targets pre-mRNA splicing factors to transcription sites in vivo. Molecular Cell, 3. pp. 697-705. ISSN 1097-2765

URL: http://www.ncbi.nlm.nih.gov/pubmed/10394358
DOI: 10.1016/S1097-2765(01)80002-2

Abstract

Biochemical evidence indicates that pre-mRNA splicing factors physically interact with the C-terminal domain of the largest subunit of RNA polymerase II. We have investigated the in vivo function of this interaction. In mammalian cells, truncation of the CTD of RNA pol II LS prevents the targeting of the splicing machinery to a transcription site. In the absence of the CTD, pre-mRNA splicing is severely reduced. The presence of unspliced RNA alone is not sufficient for the accumulation of splicing factors at the transcription site, nor for its efficient splicing. Our results demonstrate a critical role for the CTD of RNA pol II LS in the intranuclear targeting of splicing factors to transcription sites in vivo.

Item Type: Paper
Uncontrolled Keywords: Amanitins Animals Binding Sites Cell Nucleus Dichlororibofuranosylbenzimidazole Exons HeLa Cells Humans Nuclear Proteins Phosphoproteins Phosphorylation Protein Binding RNA RNA Polymerase II RNA Splicing RNA-Binding Proteins Rats Ribonucleoproteins Ribonucleoproteins, Small Nuclear Sequence Deletion Spliceosomes Transcription, Genetic
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > pre-mRNA
CSHL Authors:
Communities: CSHL labs > Spector lab
Depositing User: Matt Covey
Date Deposited: 10 Dec 2012 19:57
Last Modified: 29 Jan 2015 17:16
Related URLs:
URI: http://repository.cshl.edu/id/eprint/26345

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