Purification and biochemical characterization of interchromatin granule clusters

Mintz, P. J., Patterson, S. D., Neuwald, A. F., Spahr, C. S., Spector, D. L. (1999) Purification and biochemical characterization of interchromatin granule clusters. Embo Journal, 18 (15). pp. 4308-4320. ISSN 0261-4189

URL: http://www.ncbi.nlm.nih.gov/pubmed/10428969
DOI: 10.1093/emboj/18.15.4308

Abstract

Components of the pre-mRNA splicing machinery are localized in interchromatin granule clusters (IGCs) and perichromatin fibrils (PFs). Here we report the biochemical purification of IGCs. Approximately 75 enriched proteins were present in the IGC fraction. Protein identification employing a novel mass spectrometry strategy and peptide microsequencing identified 33 known proteins, many of which have been linked to pre-mRNA splicing, as well as numerous uncharacterized proteins. Thus far, three new protein constituents of the IGCs have been identified. One of these, a 137 kDa protein, has a striking sequence similarity over its entire length to UV-damaged DNA-binding protein, a protein associated with the hereditary disease xeroderma pigmentosum group E, and to the 160 kDa subunit of cleavage polyadenylation specificity factor. Overall, these results provide a key framework that will enable the biological functions associated with the IGCs to be elucidated.

Item Type: Paper
Uncontrolled Keywords: Amino Acid Sequence Animals Cell Nucleus Chromatin Electrophoresis, Gel, Two-Dimensional Female Liver Mass Spectrometry Mice Microscopy, Electron Molecular Sequence Data RNA Precursors RNA Splicing RNA, Messenger Sequence Homology, Amino Acid
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mRNA dynamics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > pre-mRNA
biotechnology > chromatography > protein purification
CSHL Authors:
Communities: CSHL labs > Spector lab
Depositing User: Matt Covey
Date Deposited: 10 Dec 2012 19:57
Last Modified: 29 Jan 2015 17:14
Related URLs:
URI: http://repository.cshl.edu/id/eprint/26344

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