Higher order nuclear organization: three-dimensional distribution of small nuclear ribonucleoprotein particles

Spector, D. L. (1990) Higher order nuclear organization: three-dimensional distribution of small nuclear ribonucleoprotein particles. Proceedings of the National Academy of Sciences of the United States of America, 87 (1). pp. 147-151. ISSN 0027-8424

URL: http://www.ncbi.nlm.nih.gov/pubmed/2136950

Abstract

The structural and functional organization of the cell nucleus has been investigated using three-dimensional reconstruction, immunoelectron microscopy, and high-resolution in situ autoradiography. Nuclear regions enriched in small nuclear ribonucleoprotein particles (snRNPs) form a reticular network within the nucleoplasm that extends between the nucleolar surface and the nuclear envelope. The snRNPs occupy approximately 18% of the volume of CHOC 400 cell nuclei. The in situ sites of DNA replication and transcription are complementary to, rather than coincident with, the nuclear regions concentrated in snRNPs. Based on these data a three-dimensional model of the organization of the mammalian cell nucleus is presented.

Item Type: Paper
Uncontrolled Keywords: Animals Autoradiography Cell Line Cell Nucleus Fluorescent Antibody Technique Microscopy, Electron Models, Structural RNA, Small Nuclear Ribonucleoproteins Ribonucleoproteins, Small Nuclear Thymidine Tritium Uridine
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
CSHL Authors:
Communities: CSHL labs > Spector lab
Depositing User: Matt Covey
Date Deposited: 10 Dec 2012 19:43
Last Modified: 29 Jan 2015 20:47
PMCID: PMC53217
Related URLs:
URI: http://repository.cshl.edu/id/eprint/26316

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