Spector, D. L. (1990) Higher order nuclear organization: three-dimensional distribution of small nuclear ribonucleoprotein particles. Proceedings of the National Academy of Sciences of the United States of America, 87 (1). pp. 147-151. ISSN 0027-8424
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Abstract
The structural and functional organization of the cell nucleus has been investigated using three-dimensional reconstruction, immunoelectron microscopy, and high-resolution in situ autoradiography. Nuclear regions enriched in small nuclear ribonucleoprotein particles (snRNPs) form a reticular network within the nucleoplasm that extends between the nucleolar surface and the nuclear envelope. The snRNPs occupy approximately 18% of the volume of CHOC 400 cell nuclei. The in situ sites of DNA replication and transcription are complementary to, rather than coincident with, the nuclear regions concentrated in snRNPs. Based on these data a three-dimensional model of the organization of the mammalian cell nucleus is presented.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Animals Autoradiography Cell Line Cell Nucleus Fluorescent Antibody Technique Microscopy, Electron Models, Structural RNA, Small Nuclear Ribonucleoproteins Ribonucleoproteins, Small Nuclear Thymidine Tritium Uridine |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification bioinformatics > genomics and proteomics > genetics & nucleic acid processing bioinformatics > genomics and proteomics |
| CSHL Authors: | |
| Communities: | CSHL labs > Spector lab |
| Depositing User: | Matt Covey |
| Date: | 1990 |
| Date Deposited: | 10 Dec 2012 19:43 |
| Last Modified: | 30 Sep 2019 16:37 |
| PMCID: | PMC53217 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/26316 |
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