Differential distribution of factors involved in pre-mRNA processing in the yeast cell nucleus

Potashkin, J. A., Derby, R. J., Spector, D. L. (1990) Differential distribution of factors involved in pre-mRNA processing in the yeast cell nucleus. Molecular and Cellular Biology, 10 (7). pp. 3524-3534. ISSN 0270-7306

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URL: http://www.ncbi.nlm.nih.gov/pubmed/2192258
DOI: 10.1128/MCB.10.7.3524

Abstract

The yeast cell nucleus has previously been shown to be divided into two regions by a variety of microscopic approaches. We used antibodies specific for the 2,2,7-trimethylguanosine cap structure of small nuclear ribonucleic acids (snRNAs) and for a protein component of small nuclear ribonucleoprotein particles to identify the distribution of small nuclear ribonucleoprotein particles within the yeast cell nucleus. These studies were performed with the fission yeast Schizosaccharomyces pombe and the budding yeast Saccharomyces cerevisiae. By using immunofluorescence microscopy and immunoelectron microscopy, most of the abundant snRNAs were localized to the portion of the nucleus which has heretofore been referred to as the nucleolus. This distribution of snRNAs is different from that found in mammalian cells and suggests that the nucleolar portion of the yeast nucleus contains functional domains in addition to those associated with RNA polymerase I activity.

Item Type: Paper
Uncontrolled Keywords: Antibodies Cell Nucleus Fluorescent Antibody Technique Guanosine Immunohistochemistry Microscopy, Electron RNA Precursors RNA Processing, Post-Transcriptional RNA, Small Nuclear Saccharomyces cerevisiae Schizosaccharomyces Spheroplasts
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing
bioinformatics > genomics and proteomics
organs, tissues, organelles, cell types and functions > organelles, types and functions > nucleus
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > pre-mRNA
CSHL Authors:
Communities: CSHL labs > Spector lab
Depositing User: Matt Covey
Date Deposited: 10 Dec 2012 19:43
Last Modified: 29 Jan 2015 20:46
PMCID: PMC360787
Related URLs:
URI: http://repository.cshl.edu/id/eprint/26315

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