Oncogenic Ras triggers the activation of 42-kDa mitogen-activated protein kinase in extracts of quiescent Xenopus oocytes

Shibuya, E. K., Polverino, A. J., Chang, E., Wigler, M. H., Ruderman, J. V. (October 1992) Oncogenic Ras triggers the activation of 42-kDa mitogen-activated protein kinase in extracts of quiescent Xenopus oocytes. Proceedings of the National Academy of Sciences of the United States of America, 89 (20). pp. 9831-9835. ISSN 0027-8424

URL: https://www.ncbi.nlm.nih.gov/pubmed/1384061
DOI: 10.1073/pnas.89.20.9831

Abstract

Quiescent, full-grown Xenopus oocytes, which are arrested at the G2/M border of meiosis, contain an inactive 42-kDa mitogen-activated protein kinase (p42MAPK) that is activated when oocytes are stimulated to resume the meiotic cell cycle. We have made extracts from these oocytes that respond to four cell cycle activators: oncogenic [Val12]Ras protein, clam cyclins ADELTA60 and BDELTA97, and the phosphatase inhibitor okadaic acid. All four induce the tyrosine phosphorylation and activation of p42MAPK. Both cyclins and okadaic acid, but not [Val12]Ras, also lead to activation of the endogenous cyclin B/cdc2 kinase complexes in extracts of quiescent oocytes. Using extracts prepared from cycloheximide-arrested interphase cells, we show that although p42MAPK activation can occur in response to cyclin-activated cdc2, the Ras-induced activation of p42MAPK occurs without intervening cdc2 activation. Neither the nononcogenic [Gly12]Ras nor [Val12,Arg186]-Ras, a mutant that lacks the C-terminal consensus sequence directing prenylation and subsequent membrane association, is an effective activator of p42MAPK in vitro.

Item Type: Paper
Uncontrolled Keywords: SIGNAL TRANSDUCTION signal transduction ONCOGENESIS oncogenesis CELL CYCLE cell cycle CDC2 CYCLINS cyclins MATURATION-PROMOTING FACTOR maturation promoting factor SIGNAL-REGULATED KINASES signal regulated kinases CELL-CYCLE cell cycle M-PHASE M-phase TYROSINE PHOSPHORYLATION tyrosine phosphorylation CDC2 PROTEIN protein SACCHAROMYCES-CEREVISIAE saccharomyces cerevisiae MEIOTIC MATURATION meiotic maturation GROWTH-FACTOR growth factor MAP KINASE map kinase
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > RAS
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase
organs, tissues, organelles, cell types and functions > organelles, types and functions > meiosis
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > oncogene
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > suppressor
CSHL Authors:
Communities: CSHL labs > Wigler lab
Depositing User: CSHL Librarian
Date: October 1992
Date Deposited: 11 Apr 2012 20:25
Last Modified: 02 Nov 2016 20:11
PMCID: PMC50227
Related URLs:
URI: http://repository.cshl.edu/id/eprint/26238

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