Linkage and expression of foreign DNA in cultured animal cells

Perucho, M., Wigler, M. H. (1981) Linkage and expression of foreign DNA in cultured animal cells. Cold Spring Harb Symp Quant Biol, 45 (2). pp. 829-838. ISSN 0091-7451 (Print)0091-7451 (Linking)

URL: https://www.ncbi.nlm.nih.gov/pubmed/6942952
DOI: 10.1101/SQB.1981.045.01.101

Abstract

The ability to introduce defined genes into cultured cells radically alters the prospects for understanding differentiation and growth control in higher organisms. Stable transformation of animal cells may be effected by the delivery of DNA as a calcium phosphate coprecipitate (Graham and van der Eb 1973). Since the uptake and expression of DNA is a relatively rare event, transformants are usually isolated by selection for cells that have acquired a new phenotype. For this purpose the thymidine kinase (tk)1 gene of herpes simplex virus type 1 (HSV-1) is often used as the selectable vector with tk+ recipient cells (Wigler et al. 1977). The tk+ transformants are then selected in HAT medium. Nonselectable genes may be stably introduced into cells either by direct ligation to the selectable vector (Mantei et al. 1979) or by unlinked cotransformation (Wigler et al. 1979b).

Item Type: Paper
Additional Information:
Uncontrolled Keywords: Animals Antigens Neoplasm genetics Antigens Viral genetics Antigens Viral Tumor Cells Cultured Chickens DNA genetics Gene Expression Regulation Genes Genetic Linkage Mice Mutation Rats Recombination Genetic Thymidine Kinase genetics Transformation Genetic
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA expression
Investigative techniques and equipment > cell culture
CSHL Authors:
Communities: CSHL labs > Wigler lab
Depositing User: CSHL Librarian
Date: 1981
Date Deposited: 11 Apr 2012 20:16
Last Modified: 08 Nov 2016 21:38
Related URLs:
URI: http://repository.cshl.edu/id/eprint/26236

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