Direct isolation of polymorphic markers linked to a trait by genetically directed representational difference analysis

Lisitsyn, N. A., Segre, J. A., Kusumi, K., Lisitsyn, N. M., Nadeau, J. H., Frankel, W. N., Wigler, M. H., Lander, E. S. (January 1994) Direct isolation of polymorphic markers linked to a trait by genetically directed representational difference analysis. Nat Genet, 6 (1). pp. 57-63. ISSN 1061-4036 (Print)

URL: https://www.ncbi.nlm.nih.gov/pubmed/8136836
DOI: 10.1038/ng0194-57

Abstract

We describe a technique, genetically directed representational difference analysis (GDRDA), for specifically generating genetic markers linked to a trait of interest. GDRDA is applicable, in principle, to virtually any organism, because it requires neither prior knowledge of the chromosomal location of the gene controlling the trait nor the availability of a pre-existing genetic map. Based on a subtraction technique described recently called representational difference analysis, GDRDA uses the principles of transmission genetics to create appropriate Tester and Driver samples for subtraction. We demonstrate the usefulness of GDRDA by, for example, successfully targeting three polymorphisms to an interval of less than 1 cM of the mouse nude locus of chromosome 11.

Item Type: Paper
Uncontrolled Keywords: Animals Base Sequence Chromosome Mapping Cloning Molecular Crosses Genetic DNA Primers genetics Female Genetic Markers Genetic Techniques Linkage (Genetics) Male Mice Mice Inbred Strains genetics Mice Nude genetics Molecular Probe Techniques Molecular Sequence Data Polymorphism Genetic
Subjects: diseases & disorders > cancer
Investigative techniques and equipment
bioinformatics > genomics and proteomics > annotation > map annotation
diseases & disorders > neoplasms
Investigative techniques and equipment > biomarker
organism description > animal > mammal > rodent > mouse
CSHL Authors:
Communities: CSHL labs > Wigler lab
Depositing User: CSHL Librarian
Date: January 1994
Date Deposited: 12 Apr 2012 14:34
Last Modified: 08 Nov 2016 16:49
Related URLs:
URI: http://repository.cshl.edu/id/eprint/26222

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