Mutational analysis of the cloned chicken thymidine kinase gene

Kwoh, T. J., Zipser, D., Wigler, M. H. (1983) Mutational analysis of the cloned chicken thymidine kinase gene. J Mol Appl Genet, 2 (2). pp. 191-200. ISSN 0271-6801 (Print)

URL: https://www.ncbi.nlm.nih.gov/pubmed/6875426

Abstract

We have analyzed the transcription and coding unit of the chicken thymidine kinase (tk) gene. We have constructed a library of mutant chicken tk genes by the in vitro linker insertion method of Heffron et al. A total of 125 mutations within a 3.0 kbp HindIII fragment containing the gene were isolated and mapped. The effect of each mutation upon the thymidine kinase gene was determined by measurement of the transfection efficiency in mouse Ltk- cells. The chicken tk mRNA is about 2 kb and polyadenylated. The direction of transcription was also determined. From these results, we propose a structure for the gene in which at least three small introns separate the amino acid coding region into at least four segments.

Item Type: Paper
Uncontrolled Keywords: Animals Chickens genetics Chromosome Mapping Cloning Molecular Genes Mutation Plasmids Thymidine Kinase genetics Transcription Genetic
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mutations
Investigative techniques and equipment > transfection
CSHL Authors:
Communities: CSHL labs > Wigler lab
Depositing User: CSHL Librarian
Date Deposited: 12 Apr 2012 15:38
Last Modified: 08 Nov 2016 16:55
URI: http://repository.cshl.edu/id/eprint/26212

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