Genetic and biochemical analysis of the adenylyl cyclase of Schizosaccharomyces pombe

Kawamukai, M., Ferguson, K., Wigler, M. H., Young, D. (February 1991) Genetic and biochemical analysis of the adenylyl cyclase of Schizosaccharomyces pombe. Cell Regulation, 2 (2). pp. 155-64. ISSN 1044-2030

URL: https://www.ncbi.nlm.nih.gov/pubmed/1863602

Abstract

The adenylyl cyclase gene, cyr1, of Schizosaccharomyces pombe has been cloned. We have begun an analysis of the function and regulation of adenylyl cyclase by disrupting this gene and by over-expressing all or parts of this gene in various strains. cyr1- strains are viable and contain no measurable cyclic AMP. They conjugate and sporulate under conditions that normally inhibit wild-type strains. Strains containing the cyr1 coding sequences transcribed from the strong adh1 promoter contain greatly elevated adenylyl cyclase activity, as measured in vitro, but only modestly elevated cAMP levels. Such strains conjugate and sporulate less frequently than wild-type cells upon nutrient limitation. Strains which carry the wild-type cyr1 gene but that also express high levels of the amino terminal domain of adenylyl cyclase behave much like cyr1-strains, suggesting that the amino terminal domain can bind a positive regulator. A protein that copurifies with the adenylyl cyclase of S. pombe cross-reacts to antiserum raised against the S. cerevisiae adenylyl cyclase-associated regulatory protein, CAP.

Item Type: Paper
Additional Information:
Uncontrolled Keywords: Adenylate Cyclase genetics physiology Chromosome Mapping Conjugation Genetic Gene Expression Genes Fungal Mutation Phenotype Plasmids Schizosaccharomyces enzymology genetics Spores Fungal genetics
Subjects: bioinformatics > genomics and proteomics > annotation > gene expression profiling annotation
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes
organism description > yeast
CSHL Authors:
Communities: CSHL labs > Wigler lab
Depositing User: CSHL Librarian
Date: February 1991
Date Deposited: 12 Apr 2012 14:59
Last Modified: 08 Nov 2016 21:09
PMCID: PMC361733
URI: http://repository.cshl.edu/id/eprint/26208

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