Genetic analysis of yeast RAS1 and RAS2 genes

Kataoka, T., Powers, S., McGill, C., Fasano, O., Strathern, J., Broach, J., Wigler, M. H. (June 1984) Genetic analysis of yeast RAS1 and RAS2 genes. Cell, 37 (2). pp. 437-445. ISSN 0092-8674

URL: https://www.ncbi.nlm.nih.gov/pubmed/6327067
DOI: 10.1016/0092-8674(84)90374-X

Abstract

We present a genetic analysis of RAS1 and RAS2 of S. cerevisiae, two genes that are highly homologous to mammalian ras genes. By constructing in vitro ras genes disrupted by selectable genes and introducing these by gene replacement into the respective ras loci, we have determined that neither RAS1 nor RAS2 are by themselves essential genes. However, ras1 - ras2 - spores of doubly heterozygous diploids are incapable of resuming vegetative growth. We have determined that RAS1 is located on chromosome XV, 7 cM from ade2 and 63 cM from his3; and RAS2 is located on chromosome XIV, 2 cM from met4 . We have also constructed by site-directed mutagenesis a missense mutant, RAS2val19 , which encodes valine in place of glycine at the nineteenth amino acid position, the same sort of missense mutation that is found in some transforming alleles of mammalian ras genes. Diploid yeast cells that contain this mutation are incapable of sporulating efficiently, even when they contain wild-type alleles.

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > RAS
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
organism description > yeast
CSHL Authors:
Communities: CSHL labs > Powers lab
CSHL labs > Wigler lab
Depositing User: CSHL Librarian
Date: June 1984
Date Deposited: 12 Apr 2012 18:20
Last Modified: 17 Oct 2016 16:09
Related URLs:
URI: http://repository.cshl.edu/id/eprint/26207

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