DNA sequence and characterization of the S. cerevisiae gene encoding adenylate cyclase

Kataoka, T., Broek, D., Wigler, M. H. (December 1985) DNA sequence and characterization of the S. cerevisiae gene encoding adenylate cyclase. Cell, 43 (2). pp. 493-505. ISSN 0092-8674

URL: https://www.ncbi.nlm.nih.gov/pubmed/2934138
DOI: 10.1016/0092-8674(85)90179-5

Abstract

We have cloned CYR1, the S. cerevisiae gene encoding adenylate cyclase. The DNA sequence of CYR1 can encode a protein of 2026 amino acids. This protein would contain a central region comprised of over twenty copies of a 23 amino acid repeating unit with remarkable homology to a 24 amino acid tandem repeating unit of a trace human serum glycoprotein. Gene disruption and biochemical experiments indicate that the catalytic domain of adenylate cyclase resides in the carboxyl terminal 400 amino acids. Elevated expression of adenylate cyclase suppresses the lethality that otherwise results from loss of RAS gene function in yeast. Yeast adenylate cyclase, made in E. coli, cannot be activated by added RAS protein.

Item Type: Paper
Subjects: organism description > yeast > Saccharomyces
bioinformatics > genomics and proteomics > annotation > sequence annotation
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein structure rendering
CSHL Authors:
Communities: CSHL labs > Wigler lab
Depositing User: CSHL Librarian
Date: December 1985
Date Deposited: 12 Apr 2012 18:14
Last Modified: 17 Oct 2016 16:10
Related URLs:
URI: http://repository.cshl.edu/id/eprint/26205

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