Rapid isolation of cDNA by hybridization

Hamaguchi, M., O'Connor, E. A., Chen, T., Parnell, L., McCombie, R. W., Wigler, M. H. (1998) Rapid isolation of cDNA by hybridization. Proceedings of the National Academy of Sciences of the United States of America, 95 (7). pp. 3764-9. ISSN 0027-8424

URL: http://www.ncbi.nlm.nih.gov/pubmed/9520441

Abstract

The isolation of genes from a given genomic region can be a rate-limiting step in the discovery of disease genes. We describe an approach to the isolation of cDNAs that have sequences in common with large genomic clones such as bacterial artificial chromosomes. We applied this method to loci both amplified and deleted in cancer, illustrating its usage in the identification of both oncogenes and tumor suppressor genes, respectively. The method, called rapid isolation of cDNAs by hybridization (RICH), depends on solution hybridization, enzymatic modification, and amplification/selection of sequences present in both cDNA populations and the genomic clones. The method should facilitate the development of transcription maps for large genomic clones, possibly even yeast artificial chromosomes.

Item Type: Paper
Uncontrolled Keywords: Chromosomes Bacterial DNA, Complementary genetics isolation & purification Gene Library Nucleic Acid Hybridization
Subjects: diseases & disorders > cancer
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription
bioinformatics > genomics and proteomics > annotation > map annotation
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > cDNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > oncogene
CSHL Authors:
Communities: CSHL labs > McCombie lab
CSHL labs > Wigler lab
Depositing User: CSHL Librarian
Date Deposited: 12 Apr 2012 19:09
Last Modified: 11 Apr 2013 20:31
Related URLs:
URI: http://repository.cshl.edu/id/eprint/26193

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