Novel genomic alterations and clonal evolution in chronic lymphocytic leukemia revealed by representational oligonucleotide microarray analysis (ROMA)

Grubor, V., Krasnitz, A., Troge, J. E., Meth, J. L., Lakshmi, B., Kendall, J. T., Yamrom, B., Alex, G., Pai, D., Navin, N. E., Hufnagel, L. A., Lee, Y. H., Cook, K., Allen, S. L., Rai, K. R., Damle, R. N., Calissano, C., Chiorazzi, N., Wigler, M. H., Esposito, D. (February 2009) Novel genomic alterations and clonal evolution in chronic lymphocytic leukemia revealed by representational oligonucleotide microarray analysis (ROMA). Blood, 113 (6). pp. 1294-1303. ISSN 0006-4971

URL: https://www.ncbi.nlm.nih.gov/pubmed/18922857
DOI: 10.1182/blood-2008-05-158865

Abstract

We examined copy number changes in the genomes of B cells from 58 patients with chronic lymphocytic leukemia (CLL) by using representational oligonucleotide microarray analysis (ROMA), a form of comparative genomic hybridization (CGH), at a resolution exceeding previously published studies. We observed at least 1 genomic lesion in each CLL sample and considerable variation in the number of abnormalities from case to case. Virtually all abnormalities previously reported also were observed here, most of which were indeed highly recurrent. We observed the boundaries of known events with greater clarity and identified previously undescribed lesions, some of which were recurrent. We profiled the genomes of CLL cells separated by the surface marker CD38 and found evidence of distinct subclones of CLL within the same patient. We discuss the potential applications of high-resolution CGH analysis in a clinical setting. (Blood. 2009; 113: 1294-1303)

Item Type: Paper
Uncontrolled Keywords: RECEPTOR GENE REARRANGEMENTS receptor gene rearrangements CD38 EXPRESSION expression MUTATION STATUS mutation status CHROMOSOMAL-ABERRATIONS chromosomal aberrations CYTOGENETIC ANALYSIS cytogenetic analysis DISEASE PROGRESSION disease progression IGV(H) STATUS B-CELLS B-cells SURVIVAL survival HYBRIDIZATION hybridization
Subjects: bioinformatics > genomics and proteomics > analysis and processing > microarray gene expression processing
Investigative techniques and equipment > ROMA
Investigative techniques and equipment > assays
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > copy number variants
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
diseases & disorders > cancer > cancer types > leukemia
CSHL Authors:
Communities: CSHL labs > Krasnitz lab
CSHL labs > Wigler lab
Watson School > Publications
Depositing User: CSHL Librarian
Date: 5 February 2009
Date Deposited: 12 Apr 2012 18:32
Last Modified: 07 Jan 2019 16:54
Related URLs:
URI: http://repository.cshl.edu/id/eprint/26189

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving