New human transforming genes detected by a tumorigenicity assay

Fasano, O., Birnbaum, D., Edlund, L., Fogh, J., Wigler, M. H. (September 1984) New human transforming genes detected by a tumorigenicity assay. Molecular & Cellular Biology, 4 (9). pp. 1695-1705.

[img]
Preview
PDF (Paper)
New human transforming genes detected.pdf - Published Version

Download (2679Kb) | Preview
URL: https://www.ncbi.nlm.nih.gov/pubmed/6092933
DOI: 10.1128/MCB.4.9.1695

Abstract

A sensitive bioassay for transforming genes based on the tumorigenicity of cotransfected NIH3T3 cells in nude mice was developed. The assay differs substantially from the NIH3T3 focus assay. Using it, the transfer of 3 transforming genes from the DNA of MCF-7, a human mammary carcinoma cell line was detected. One of these is N-ras, which is amplified in MCF-7 DNA. The other two, which were called mcf2 and mcf3, do not appear to be related to known oncogenes. Their transfer could not be detected by using the NIH3T3 focus assay. Whether either mcf2 or mcf3 is associated with genetic abnormalities in MCF-7 cells is not yet known.

Item Type: Paper
Subjects: Investigative techniques and equipment > assays
organs, tissues, organelles, cell types and functions > organs types and functions > breast
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > oncogene
CSHL Authors:
Communities: CSHL labs > Wigler lab
Depositing User: CSHL Librarian
Date: September 1984
Date Deposited: 13 Apr 2012 20:50
Last Modified: 04 Nov 2016 20:29
PMCID: PMC368975
Related URLs:
URI: http://repository.cshl.edu/id/eprint/26177

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving