Analysis of the transforming potential of the human H-ras gene by random mutagenesis

Fasano, O., Aldrich, T., Tamanoi, F., Taparowsky, E., Furth, M., Wigler, M. H. (1984) Analysis of the transforming potential of the human H-ras gene by random mutagenesis. Proceedings of the National Academy of Sciences of the United States of America, 81 (13). pp. 4008-4012.

URL: https://www.ncbi.nlm.nih.gov/pubmed/6330729
DOI: 10.1073/pnas.81.13.4008

Abstract

Some tumor cells contain mutant ras genes that are capable of transforming NIH 3T3 cells. Those genes that were analyzed arise from the wild-type, non-transforming ras genes by mutations producing single amino acid substitutions at position 12 or 61 of the encoded protein. Random bisulfite-induced mutagenesis was performed on the cloned wild-type human H-ras gene to find if mutations at other positions can activate the transforming potential of that gene. Most mutations are not activating, but mutations that specify single amino acid substitutions at position 12, 13, 59, or 63 if the encoded protein do activate the transforming potential of the H-ras gene. Some, but not all, mutant ras proteins show an altered electrophoretic mobility in NaDodSO4[sodium dodecyl sulfate]/polyacrylamide gels.

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > RAS
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mutations
CSHL Authors:
Communities: CSHL labs > Wigler lab
Depositing User: CSHL Librarian
Date Deposited: 13 Apr 2012 20:41
Last Modified: 09 Nov 2016 17:21
PMCID: PMC345357
Related URLs:
URI: http://repository.cshl.edu/id/eprint/26176

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