cAMP-independent control of sporulation, glycogen metabolism, and heat shock resistance in S. cerevisiae

Cameron, S., Levin, L., Zoller, M., Wigler, M. H. (May 1988) cAMP-independent control of sporulation, glycogen metabolism, and heat shock resistance in S. cerevisiae. Cell, 53 (4). pp. 555-66. ISSN 0092-8674

URL: https://www.ncbi.nlm.nih.gov/pubmed/2836063
DOI: 10.1016/0092-8674(88)90572-7

Abstract

Genes encoding the regulatory (BCY1) and catalytic (TPK1, TPK2, and TPK3) subunits of the cAMP-dependent protein kinase (cAPK) are found in S. cerevisiae. bcy1- yeast strains do not respond properly to nutrient conditions. Unlike wild type, bcy1- strains do not accumulate glycogen, form spores, or become resistant to heat shock when nutrient limited. We have isolated mutant TPK genes that suppress all of the bcy1- defects. The mutant TPK genes appear to encode functionally attenuated catalytic subunits of the cAPK. bcy1- yeast strains containing the mutant TPK genes respond appropriately to nutrient conditions, even in the absence of CDC25, both RAS genes, or CYR1. Together, these genes encode the known components of the cAMP-generating machinery. The results indicate that cAMP-independent mechanisms must exist for regulating glycogen accumulation, sporulation, and the acquisition of thermotolerance in S. cerevisiae.

Item Type: Paper
Additional Information:
Uncontrolled Keywords: Alleles Carrier Proteins genetics Cyclic AMP metabolism Diploidy Genes Fungal Genes ras Genetic Complementation Test Genotype Glycogen metabolism Heat Intracellular Signaling Peptides and Proteins Mutation Phenotype Protein Kinases genetics metabolism Saccharomyces cerevisiae enzymology genetics growth & development metabolism physiology Spores Fungal physiology
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > RAS
organism description > yeast > Saccharomyces
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase
CSHL Authors:
Communities: CSHL labs > Wigler lab
Depositing User: CSHL Librarian
Date: 20 May 1988
Date Deposited: 19 Apr 2012 14:05
Last Modified: 18 Nov 2016 17:10
Related URLs:
URI: http://repository.cshl.edu/id/eprint/26167

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