A family of human phosphodiesterases homologous to the dunce learning and memory gene product of Drosophila melanogaster are potential targets for antidepressant drugs

Bolger, G., Michaeli, T., Martins, T., St John, T., Steiner, B., Rodgers, L., Riggs, M., Wigler, M. H., Ferguson, K. (October 1993) A family of human phosphodiesterases homologous to the dunce learning and memory gene product of Drosophila melanogaster are potential targets for antidepressant drugs. Mol Cell Biol, 13 (10). pp. 6558-71. ISSN 0270-7306 (Print)

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URL: https://www.ncbi.nlm.nih.gov/pubmed/8413254
DOI: 10.1128/MCB.13.10.6558

Abstract

We have isolated cDNAs for four human genes (DPDE1 through DPDE4) closely related to the dnc learning and memory locus of Drosophila melanogaster. The deduced amino acid sequences of the Drosophila and human proteins have considerable homology, extending beyond the putative catalytic region to include two novel, highly conserved, upstream conserved regions (UCR1 and UCR2). The upstream conserved regions are located in the amino-terminal regions of the proteins and appear to be unique to these genes. Polymerase chain reaction analysis suggested that these genes encoded the only homologs of dnc in the human genome. Three of the four genes were expressed in Saccharomyces cerevisiae and shown to encode cyclic AMP-specific phosphodiesterases. The products of the expressed genes displayed the pattern of sensitivity to inhibitors expected for members of the type IV, cyclic AMP-specific class of phosphodiesterases. Each of the four genes demonstrated a distinctive pattern of expression in RNA from human cell lines.

Item Type: Paper
Uncontrolled Keywords: 3',5'-Cyclic-Nucleotide Phosphodiesterase/ drug effects genetics metabolism Alternative Splicing Amino Acid Sequence Animals Antidepressive Agents pharmacology Base Sequence Blotting Northern Cloning Molecular Dna Drosophila melanogaster Humans Learning Memory Molecular Sequence Data Organ Specificity genetics Polymerase Chain Reaction Sequence Alignment Sequence Homology Sequence Homology, Amino Acid Transcription Genetic
Subjects: organism description > animal > insect > Drosophila
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > cDNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
organism description > animal behavior > learning
organism description > animal behavior > memory
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein characterization
CSHL Authors:
Communities: CSHL labs > Wigler lab
Depositing User: CSHL Librarian
Date: October 1993
Date Deposited: 19 Apr 2012 15:25
Last Modified: 04 Nov 2016 19:59
PMCID: PMC364715
Related URLs:
URI: http://repository.cshl.edu/id/eprint/26162

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