Genetic analysis of mammalian GAP expressed in yeast

Ballester, R., Michaeli, T., Ferguson, K., Xu, H. P., McCormick, F., Wigler, M. H. (November 1989) Genetic analysis of mammalian GAP expressed in yeast. Cell, 59 (4). pp. 681-6. ISSN 0092-8674

URL: https://www.ncbi.nlm.nih.gov/pubmed/2684416
DOI: 10.1016/0092-8674(89)90014-7

Abstract

We have designed a vector to express the mammalian GAP protein in the yeast S. cerevisiae. When expressed in yeast, GAP inhibits the function of the human H-rasgly12 protein, but not that of the H-rasval12 protein, and complements the loss of IRA1. IRA1 is a yeast gene that encodes a protein with homology to GAP and acts upstream of RAS. Mammalian GAP can therefore function in yeast and interact with yeast RAS. Because expression of GAP complements ira1-mutants, we propose that GAP shares some biochemical functions with IRA1. Other studies indicate that IRA1 controls the level of RAS activity, presumably by regulating GTP hydrolysis. By analogy, we propose that GAP may play a similar role.

Item Type: Paper
Additional Information:
Uncontrolled Keywords: Cloning, Molecular methods Escherichia coli genetics GTPase Activating Proteins Gene Expression Genes Structural Genes ras Genetic Vectors Heat Plasmids Protein Biosynthesis Proteins genetics Restriction Mapping Saccharomyces cerevisiae genetics ras GTPase-Activating Proteins
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > RAS
organism description > yeast > Saccharomyces
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein characterization
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein expression
CSHL Authors:
Communities: CSHL labs > Wigler lab
Depositing User: CSHL Librarian
Date: 17 November 1989
Date Deposited: 19 Apr 2012 16:26
Last Modified: 18 Nov 2016 17:05
Related URLs:
URI: http://repository.cshl.edu/id/eprint/26152

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