The NF1 locus encodes a protein functionally related to mammalian GAP and yeast IRA proteins

Ballester, R., Marchuk, D., Boguski, M., Saulino, A., Letcher, R., Wigler, M. H., Collins, F. (November 1990) The NF1 locus encodes a protein functionally related to mammalian GAP and yeast IRA proteins. Cell, 63 (4). pp. 851-9. ISSN 0092-8674

URL: https://www.ncbi.nlm.nih.gov/pubmed/2121371
DOI: 10.1016/0092-8674(90)90151-4

Abstract

The von Recklinghausen neurofibromatosis locus, NF1, encodes a protein with homology restricted to the catalytic region of the RAS GTPase-activating protein, GAP, and with extensive homology to the IRA1 and IRA2 gene products of the yeast S. cerevisiae. A segment of the NF1 cDNA gene, expressed in yeast, can complement loss of IRA function and can inhibit both wild-type and mutant activated human H-ras genes that are coexpressed in yeast. Yeast expressing the NF1 segment have increased H-ras GTPase-stimulating activity. These studies indicate that the NF1 gene product can interact with RAS proteins and demonstrate structural and functional similarities and differences among the GAP, IRA1, IRA2, and NF1 proteins.

Item Type: Paper
Additional Information:
Uncontrolled Keywords: Amino Acid Sequence Base Sequence Fungal Proteins genetics GTP Phosphohydrolases metabolism GTPase-Activating Proteins Genetic Complementation Test Genetic Vectors Humans Molecular Sequence Data Neurofibromatosis 1 genetics Neurofibromin 1 Oligonucleotide Probes Oncogene Protein p21(ras) genetics RAS Plasmids Proteins genetics Saccharomyces cerevisiae genetics Saccharomyces cerevisiae Proteins Sequence Homology, Nucleic Acid ras GTPase-Activating Proteins
Subjects: diseases & disorders > congenital hereditary genetic diseases
diseases & disorders > nervous system diseases and disorders
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > RAS
organism description > yeast > Saccharomyces
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein characterization
CSHL Authors:
Communities: CSHL labs > Wigler lab
Depositing User: CSHL Librarian
Date: 16 November 1990
Date Deposited: 19 Apr 2012 16:20
Last Modified: 18 Nov 2016 16:36
Related URLs:
URI: http://repository.cshl.edu/id/eprint/26151

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