Sorting of small RNAs into Arabidopsis argonaute complexes is directed by the 5' terminal nucleotide

Mi, S. J., Cai, T., Hu, Y. G., Chen, Y., Hodges, E., Ni, F. R., Wu, L., Li, S., Zhou, H., Long, C. Z., Chen, S., Hannon, G. J., Qi, Y. J. (April 2008) Sorting of small RNAs into Arabidopsis argonaute complexes is directed by the 5' terminal nucleotide. Cell, 133 (1). pp. 116-127. ISSN 0092-8674

URL: https://www.ncbi.nlm.nih.gov/pubmed/18342361
DOI: 10.1016/j.cell.2008.02.034

Abstract

Argonaute (AGO) proteins recruit small RNAs to form the core of RNAi effector complexes. Arabidopsis encodes ten AGO proteins and a large network of small RNAs. How these small RNAs are sorted into specific AGO complexes remains largely unknown. We have cataloged small RNAs resident in four AGO complexes. We found that AGO2 and AGO4 preferentially recruit small RNAs with a 5 ' terminal adenosine, whereas AGO1 harbors microRNAs (miRNAs) that favor a 5 ' terminal uridine. AGO5 predominantly binds small RNAs that initiate with cytosine. Changing the 5 ' terminal nucleotide of an miRNA predictably redirected it into a different AGO complex and alters its biological activity. These results reveal a role for small RNA sequences in assorting among AGO complexes. This suggests that specialization of AGO complexes might involve remodeling the 5 ' end-binding pocket to accept certain small RNA sequences, perhaps explaining the evolutionary drive for miRNAs to initiate with uridine.

Item Type: Paper
Uncontrolled Keywords: TRANS ACTING SIRNAS Trans acting sirnas C ELEGANS C elegans ANTISENSE TRANSCRIPTS Antisense transcripts ENDOGENOUS Endogenous SIRNAS Sirnas PROCESSING CENTER Processing center DNA METHYLATION DNA methylation MESSENGER RNAS Messenger RNAs MICRORNAS Micrornas BIOGENESIS Biogenesis DROSOPHILA Drosophila
Subjects: organism description > plant > Arabidopsis
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNAi
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > argonaute proteins
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > sRNA
CSHL Authors:
Communities: CSHL labs > Hannon lab
Depositing User: Editor Margaret Fantz
Date: 4 April 2008
Date Deposited: 10 Apr 2012 18:11
Last Modified: 02 Dec 2016 17:22
PMCID: PMC2981139
Related URLs:
URI: http://repository.cshl.edu/id/eprint/26127

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