A role for the P-body component GW182 in microRNA function

Liu, J. D., Rivas, F. V., Wohlschlegel, J., Yates, J. R., Parker, R., Hannon, G. J. (December 2005) A role for the P-body component GW182 in microRNA function. Nature Cell Biology, 7 (12). pp. 1261-1266. ISSN 1465-7392

URL: http://www.ncbi.nlm.nih.gov/pubmed/16284623
DOI: 10.1038/ncb1333

Abstract

In animals, the majority of microRNAs regulate gene expression through the RNA interference (RNAi) machinery without inducing small-interfering RNA (siRNA)-directed mRNA cleavage(1). Thus, the mechanisms by which microRNAs repress their targets have remained elusive. Recently, Argonaute proteins, which are key RNAi effector components, and their target mRNAs were shown to localize to cytoplasmic foci known as P-bodies or GW-bodies(2,3). Here, we show that the Argonaute proteins physically interact with a key P-/GW-body subunit, GW182. Silencing of GW182 delocalizes resident P-/GW-body proteins and impairs the silencing of microRNA reporters. Moreover, mutations that prevent Argonaute proteins from localizing in P-/GW-bodies prevent translational repression of mRNAs even when Argonaute is tethered to its target in a siRNA-independent fashion. Thus, our results support a functional link between cytoplasmic P-bodies and the ability of a microRNA to repress expression of a target mRNA.

Item Type: Paper
Uncontrolled Keywords: MESSENGER RNA DEGRADATION messenger rna degradation PROCESSING BODIES processing bodies PROTEIN SYNTHESIS protein synthesis GW gw BODIES bodies C ELEGANS c elegans C elegans TRANSLATION translation ARGONAUTE 2 argonaute 2 COMPLEX complex MIRNAS mirnas miRNA's miRNA TARGET target
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > RNAi
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > argonaute proteins
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > miRNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > miRNA
CSHL Authors:
Communities: CSHL labs > Hannon lab
Depositing User: Editor Margaret Fantz
Date: December 2005
Date Deposited: 16 Apr 2012 18:49
Last Modified: 01 Mar 2013 20:17
PMCID: PMC1804202
Related URLs:
URI: http://repository.cshl.edu/id/eprint/26116

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving