Complex synthetic chemical libraries indexed with molecular tags

Ohlmeyer, M. H. J., Swanson, R. N., Dillard, L. W., Reader, J. C., Asouline, G., Kobayashi, R., Wigler, M. H., Still, W. C. (December 1993) Complex synthetic chemical libraries indexed with molecular tags. Proceedings of the National Academy of Sciences of the United States of America, 90 (23). pp. 10922-10926. ISSN 0027-8424

URL: https://www.ncbi.nlm.nih.gov/pubmed/7504286

Abstract

Combinatorial methods of chemical synthesis allow the creation of molecular libraries having immense diversity. The utility of such libraries is dependent upon identifying the structures of the molecules so prepared. We describe the construction of a peptide combinatorial library, having 117,649 different members, synthesized on beads and indexed with inert chemical tags. These tags are used as a binary code to record the reaction history of each bead. The code can be read directly from a single bead by electron capture capillary gas chromatography. We demonstrate the correct selection of members of the library on the basis of binding to a monoclonal antibody.

Item Type: Paper
Uncontrolled Keywords: COMBINATORIAL CHEMISTRY combinatorial chemistry ENCODED LIBRARIES encoded libraries PEPTIDES peptides ANTIBODY antibody RECOGNITION recognition PEPTIDE-SYNTHESIS peptide synthesis
Subjects: bioinformatics > genomics and proteomics > design > protein network design > peptide design
bioinformatics > genomics and proteomics > design > protein network design
bioinformatics > genomics and proteomics > databases > databases
CSHL Authors:
Communities: CSHL labs > Wigler lab
Depositing User: CSHL Librarian
Date: December 1993
Date Deposited: 05 Apr 2012 15:57
Last Modified: 02 Nov 2016 19:29
PMCID: PMC47893
Related URLs:
URI: http://repository.cshl.edu/id/eprint/26030

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