Genetic and Physical Linkage of Exogenous Sequences in Transformed-Cells

Perucho, M., Hanahan, D., Wigler, M. H. (1980) Genetic and Physical Linkage of Exogenous Sequences in Transformed-Cells. Cell, 22 (1). pp. 309-317. ISSN 0092-8674

URL: https://www.ncbi.nlm.nih.gov/pubmed/6253083
DOI: 10.1016/0092-8674(80)90178-6

Abstract

The calcium phosphate precipitation method of Graham and van der Eb (1973) is an efficient means of introducing DNA into cultured animal cells. Cells which incorporate one selectable marker are also likely to incorporate sequences from the carrier DNA. Both selected and unselected markers are found integrated in the high molecular weight nuclear DNA of the host. In the present study, we demonstrate that exogenously acquired sequences are gentically linked, segregating and amplifying coordinately, and that their flanking sequences derive primarily from the carrier species rather than the host species. Based on these results, we propose that, upon transformation, the host cell ligates incorporated DNA into a large concatameric structure which may at times be as large as 2000 kilobases. From blotting data alone we cannot determine whether this structure is chromosomal or extrachromosomal in location.

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics > annotation > sequence annotation
CSHL Authors:
Communities: CSHL labs > Wigler lab
Depositing User: CSHL Librarian
Date: 1980
Date Deposited: 05 Apr 2012 16:57
Last Modified: 02 Nov 2016 16:54
Related URLs:
URI: http://repository.cshl.edu/id/eprint/26026

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