RAM, a gene of yeast required for a functional modification of RAS proteins and for production of mating pheromone a-factor

Powers, S., Michaelis, S., Broek, D., Ann-A, S. S., Field, J., Herskowitz, I., Wigler, M. H. (1986) RAM, a gene of yeast required for a functional modification of RAS proteins and for production of mating pheromone a-factor. Cell, 47 (3). pp. 413-422.

URL: https://www.ncbi.nlm.nih.gov/pubmed/3533274
DOI: 10.1016/0092-8674(86)90598-2

Abstract

We have identified a gene (SUPH) of S. cerevisiae that is requried for both RAS function and mating by cells of a mating type. supH is allelic to ste16, a gene required for the production of the mating pheromone a-factor. Both RAS and a-factor coding sequences terminate with the potential acyltransferase recognition sequence Cys-A-A-X, where A is an aliphatic amino acid. Mutations in SUPH-STE16 prevent the membrane localization and maturation of RAS protein, as well as the fatty acid acylation of it and other membrane proteins. We propose the designation RAM (RAS protein and a-factor maturation function) for SUPH and STE16. RAM may encode an enzyme responsible for the modification and membrane localization of proteins with this C-terminal sequence.

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > RAS
organism description > yeast > Saccharomyces
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
CSHL Authors:
Communities: CSHL labs > Powers lab
CSHL labs > Wigler lab
Depositing User: CSHL Librarian
Date: 1986
Date Deposited: 05 Apr 2012 16:32
Last Modified: 18 Nov 2016 16:37
Related URLs:
URI: http://repository.cshl.edu/id/eprint/26021

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