Dominant yeast and mammalian RAS mutants that interfere with the CDC25-dependent activation of wild-type RAS in Saccharomyces cerevisiae

Powers, S., O'Neill, K., Wigler, M. H. (February 1989) Dominant yeast and mammalian RAS mutants that interfere with the CDC25-dependent activation of wild-type RAS in Saccharomyces cerevisiae. Molecular & Cellular Biology, 9 (2). pp. 390-5. ISSN 0270-7306

[img]
Preview
PDF (Paper)
Dominant yeast and mammalian RAS mutants.pdf - Published Version

Download (1407Kb) | Preview
URL: https://www.ncbi.nlm.nih.gov/pubmed/2651897
DOI: 10.1128/MCB.9.2.390

Abstract

Two mutant alleles of RAS2 were discovered that dominantly interfere with wild-type RAS function in the yeast Saccharomyces cerevisiae. An amino acid substitution which caused the dominant interference was an alanine for glycine at position 22 or a proline for alanine at position 25. Analogous mutations in human H-ras also dominantly inhibited RAS function when expressed in yeast cells. The inhibitory effects of the mutant RAS2 or H-ras genes could be overcome by overexpression of CDC25, but only in the presence of wild-type RAS. These results suggest that these mutant RAS genes interfere with the normal interaction of RAS and CDC25 proteins and suggest that this interaction is direct and has evolutionarily conserved features.

Item Type: Paper
Uncontrolled Keywords: Alleles Cell Cycle Proteins Evolution Fungal Proteins genetics Genes Dominant Genes Fungal Genes ras Humans Mutation Saccharomyces cerevisiae genetics Saccharomyces cerevisiae Proteins Temperature ras Proteins ras-GRF1
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > RAS
organism description > yeast > Saccharomyces
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
CSHL Authors:
Communities: CSHL labs > Powers lab
CSHL labs > Wigler lab
Depositing User: CSHL Librarian
Date: February 1989
Date Deposited: 05 Apr 2012 16:29
Last Modified: 04 Nov 2016 20:41
PMCID: PMC362613
Related URLs:
URI: http://repository.cshl.edu/id/eprint/26020

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving