Cloning and characterization of the high-affinity cAMP phosphodiesterase of Saccharomyces cerevisiae

Sass, P., Field, J., Nikawa, J., Toda, T., Wigler, M. H. (1986) Cloning and characterization of the high-affinity cAMP phosphodiesterase of Saccharomyces cerevisiae. Proceedings of the National Academy of Sciences of the United States of America, 83 (24). pp. 9303-9307.

URL: https://www.ncbi.nlm.nih.gov/pubmed/3025832
DOI: 10.1073/pnas.83.24.9303

Abstract

A gene, PDE2, has been cloned from the yeast Saccharomyces cerevisiae that, when present in high copy, reverses the phenotypic effects of RAS2Val19, a mutant form of the RAS2 gene that renders yeast cells sensitive to heat shock and starvation. It has previously been shown that the RAS proteins are potent activators of yeast adenylate cyclase. We report here that PDE2 encodes a high-affinity cAMP phosphodiesterase that shares sequence homology with animal cell phosphodiesterases. These results therefore imply that the effects of RAS2Val119 are mediated through its changes in cAMP concentration.

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > RAS
organism description > yeast > Saccharomyces
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
CSHL Authors:
Communities: CSHL labs > Wigler lab
Depositing User: CSHL Librarian
Date: 1986
Date Deposited: 05 Apr 2012 18:06
Last Modified: 09 Nov 2016 20:48
PMCID: PMC387126
Related URLs:
URI: http://repository.cshl.edu/id/eprint/26014

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